Characterization of monoclonal antibodies against the second-generation schizonts of Leucocytozoon caulleryi.
Characterization of monoclonal antibodies against the second-generation schizonts of Leucocytozoon caulleryi.
复制标题
针对第二代白细胞虫裂殖体的单克隆抗体的表征。
DOI：
--
复制
发表时间：
2002
影响因子：
1.2
通讯作者：
Y. Fujisaki
中科院分区：
文献类型：
--
作者：
T. Gotanda;M. Doi;Y. Eiguchi;Yoshio Tanaka;Shigeki Kobayashi;Y. Fujisaki
制备了针对卡勒利白细胞原虫（L. caulleryi）第二代分裂体的单克隆抗体R1和M5。免疫荧光显微镜观察两种抗体均能与第二代裂殖菌的膜蛋白和内部结构蛋白发生反应。采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和Western blotting分析第二代分裂体（2GS）抗原的分子量。在还原条件下，SDS-PAGE在2GS抗原中检测到至少40个蛋白条带，范围在10 ~ 270 kDa之间。单抗R1与2GS抗原中150 ~ 268 kDa的多肽发生反应，而单抗M5与66 kDa的多肽发生反应。用单克隆抗体R1和M5亲和层析分离2GS抗原蛋白，可保护鸡免受氏乳杆菌孢子虫的侵袭。这些结果表明，单克隆抗体R1和M5能够识别大肠杆菌的2GS抗原。
Monoclonal antibodies (MAbs), R1 and M5, were established against the second-generation schizont of Leucocytozoon caulleryi (L. caulleryi). Both antibodies reacted to membrane and internal structure proteins of the second-generation schizont by immunofluorescence microscopy. Molecular weight of the second-generation schizont (2GS) antigen was analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. At least 40 protein bands were detected in 2GS antigen by SDS-PAGE under reduced condition and ranged from 10 to 270 kDa. MAb R1 reacted to polypeptides of 150-268 kDa in 2GS antigen, whereas MAb M5 did with that of 66 kDa. Injection with a protein of 2GS antigen fractionated by affinity chromatography using MAbs R1 and M5 protected chickens against challenge with sporozoites of L. caulleryi. These results suggest that MAbs, R1 and M5, recognize 2GS antigen of L caulleryi.
与无性恶性疟原虫高分子量抗原反应的两种单克隆抗体的表征。
影响因子：
1.5
作者：
Handunnetti,SM;Pasloske,BL;vanSchravendijk,MR;Aguiar,JC;Taraschi,TF;Gormley,JA;Howard,RJ
通讯作者：
Howard,RJ