Hyperpolarized c-13 spectroscopic imaging of the TRAMP mouse at 3T - Initial experience
Hyperpolarized c-13 spectroscopic imaging of the TRAMP mouse at 3T - Initial experience
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DOI：
10.1002/mrm.21256
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发表时间：
2007-12-01
影响因子：
3.3
通讯作者：
Vigneron, Daniel B.
中科院分区：
文献类型：
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作者：
Chen, Albert P.;Albers, Mark J.;Vigneron, Daniel B.
转基因小鼠前列腺腺癌（TRAMP）小鼠是一种被充分研究的前列腺癌小鼠模型，其组织病理学和疾病进展与人类疾病相似。为了研究正常前列腺上皮细胞和前列腺肿瘤细胞之间的细胞生物能量学差异，在TRAMP模型中使用非质子核（如C-13）进行体内磁共振光谱（MRS）研究将非常有用。最近发展了一种在溶液中保持动态核极化（DNP）的方法，允许在注射超极化C-13剂后获得高信噪比（SNR）的C-13 MRI或MRSI数据。在这项转基因小鼠研究中，该方法采用双自旋回波（DSE）脉冲序列，包括小尖端角激励射频脉冲、双曲正割重聚焦脉冲和飞回回波平面读取轨迹，在0.135 cm(3)标称空间分辨率下对C-13丙酮酸酯（pyr）及其代谢产物进行快速（10-14 s）磁共振成像。在原发性和转移性肿瘤中均观察到升高的C-13乳酸（lac），证明了用DNP超极化C-13 MRSI研究体内细胞生物能量学的可行性。
The transgenic adenocarcinoma of mouse prostate (TRAMP) mouse is a well-studied murine model of prostate cancer with histopathology and disease progression that mimic the human disease. To investigate differences in cellular bioenergetics between normal prostate epithelial cells and prostate tumor cells, in vivo MR spectroscopic (MRS) studies with non-proton nuclei, such as C-13, in the TRAMP model would be extremely useful. The recent development of a method for retaining dynamic nuclear polarization (DNP) in solution permits high signal-to-noise ratio (SNR) C-13 MRI or MRSI data to be obtained following injection of a hyperpolarized C-13 agent. In this transgenic mouse study, this method was applied using a double spin-echo (DSE) pulse sequence with a small-tip-angle excitation RF pulse, hyperbolic-secant refocusing pulses, and a flyback echo-planar readout trajectory for fast (10-14 s) MRSI of C-13 pyruvate (pyr) and its metabolic products at 0.135 cm(3) nominal spatial resolution. Elevated C-13 lactate (lac) was observed in both primary and metastatic tumors, demonstrating the feasibility of studying cellular bioenergetics in vivo with DNP hyperpolarized C-13 MRSI.