Relationship between monokaryotic growth rate and mating type in the edible basidiomycete Pleurotus ostreatus
Relationship between monokaryotic growth rate and mating type in the edible basidiomycete Pleurotus ostreatus
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DOI：
10.1128/aem.67.8.3385-3390.2001
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发表时间：
2001-08-01
影响因子：
4.4
通讯作者：
Ramírez, L
中科院分区：
文献类型：
--
作者：
Larraya, LM;Pérez, G;Ramírez, L
平菇是一种工业化生产的异宗配合同担子菌，其交配受双因子四极遗传系统控制。两个交配位点（matA和matB）控制不同的步骤菌丝融合，核迁移，核分选的发病和进展的dilatum增长。先前的研究表明，存在于matB基因座的等位基因的分离不同于单个基因座的预期分离，因为（i）新的非亲本B等位基因出现在后代中，以及（ii）靠近该交配基因座的基因组区域的分离存在畸变。在这项研究中，我们通过使用遗传学方法进行这些观察，该方法基于与matB基因座连锁的分子标记的鉴定，该分子标记使我们能够将其解剖成两个遗传连锁的亚基（matB α和matB β），并将特定matB α和matA等位基因的存在与单核细胞生长速率的差异相关联。这些分子标记的可用性和单核体中生长速率的交配型依赖性可以有助于标记辅助选择用于构建双核体的快速生长单核体，所述双核体能够比负责减少该真菌的工业产量的竞争对手更快地在基质上定殖。
The edible fungus Pleurotus ostreatus (oyster mushroom) is an industrially produced heterothallic homobasidiomycete whose mating is controlled by a bifactorial tetrapolar genetic system. Two mating loci (matA and matB) control different steps of hyphal fusion, nuclear migration, and nuclear sorting during the onset and progress of the dikaryotic growth. Previous studies have shown that the segregation of the alleles present at the matB locus differs from that expected for a single locus because (i) new nonparental B alleles appeared in the progeny and (ii) there was a distortion in the segregation of the genomic regions close to this mating locus. In this study, we pursued these observations by using a genetic approach based on the identification of molecular markers linked to the matB locus that allowed us to dissect it into two genetically linked subunits (matB alpha and matB beta) and to correlate the presence of specific matB alpha and matA alleles with differences in monokaryotic growth rate. The availability of these molecular markers and the mating type dependence of growth rate in monokaryons can be helpful for marker-assisted selection of fast-growing monokaryons to be used in the construction of dikaryons able to colonize the substrate faster than the competitors responsible for reductions in the industrial yield of this fungus.