Cellular quiescence induced by contact inhibition or serum withdrawal in C3H10T1/2 cells
Cellular quiescence induced by contact inhibition or serum withdrawal in C3H10T1/2 cells
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DOI：
10.1111/j.1365-2184.2005.00334.x
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发表时间：
2005-04-01
影响因子：
8.5
通讯作者：
Janik, P
中科院分区：
文献类型：
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作者：
Gos, M;Miloszewska, J;Janik, P
无论是汇合或血清撤回可能会导致生长停滞的培养非转化细胞。在这里，我们比较了稀疏的人口和汇合的C3 H10 T1/2细胞和无血清培养基。以下增殖相关的终点进行了检查：细胞周期分布，Ki-67抗原的存在，冯希佩尔-林道（VHL）蛋白的水平，和基因表达，使用微阵列方法确定。在稀疏/对数培养中，在血清撤除后，G（0）/G（1）期细胞的分数从55%增加到85%。此外，Ki-67阳性细胞的分数从89%下降到47%。在汇合培养物中，大多数细胞（80%）处于G（0）/G（1）期，只有25-30%的细胞为Ki-67阳性，无论是否存在血清。在这两个血清剥夺和接触抑制文化，基因表达的显着和明显的变化进行了观察。稀疏培养的细胞的血清剥夺导致几种转录因子的显著过度表达，而汇合的细胞显示编码Wnt 6、uPar、Tdag 51、Egr 1、Tdag 1a和Mor 1的基因的表达升高。这些结果表明，接触抑制和血清撤回导致细胞静止通过不同的遗传和分子机制。
Either confluence or serum withdrawal may cause growth arrest of cultured non-transformed cells. Here, we compared sparsely populated and confluent C3H10T1/2 cells with and without serum-containing medium. The following proliferation-relevant end points were examined: cell-cycle distribution, Ki-67 antigen presence, the level of the von Hippel-Lindau (VHL) protein, and gene expression, determined using a microarray approach. In sparse/logarithmic cultures, the fraction of cells in G(0)/G(1) phase increased from 55 to 85% following serum withdrawal. Moreover, the fraction of Ki-67 positive cells dropped from 89 to 47%. In confluent cultures, the majority of cells (80%) were in G(0)/G(1) phase and only 25-30% were Ki-67 positive, regardless of serum presence. In both serum-deprived and contact-inhibited cultures, significant and distinct changes in gene expression were observed. Serum deprivation of sparsely cultured cells resulted in significant over-expression of several transcription factors, while confluent cells showed elevated expression of genes coding for Wnt6, uPar, Tdag51, Egr1, Ini1a and Mor1. These results indicate that contact inhibition and serum withdrawal lead to cellular quiescence through distinct genetic and molecular mechanisms.