Artificial insemination outcomes in beef females using bovine sperm with a detectable fertility-associated antigen.
Artificial insemination outcomes in beef females using bovine sperm with a detectable fertility-associated antigen.
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使用带有可检测的生育相关抗原的牛精子对雌性肉牛进行人工授精。
DOI：
10.2527/2000.784795x
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发表时间：
2000
影响因子：
3.3
通讯作者：
R. Ax
中科院分区：
文献类型：
--
作者：
L. Sprott;M. D. Harris;D. Forrest;J. Young;H. Zhang;J. Oyarzo;M. Bellin;R. Ax
在这项研究中，对来自 25 头已通过繁殖健全性评估的公牛的精液样本进行了分析，以确定精子膜上是否存在 31 kDa 的蛋白质，即生育相关抗原 (FAA)。十八头公牛精子上有 FAA（FAA 阳性），七头公牛精子上没有 FAA（FAA 阴性）。将每头公牛的一次射精延长并与 25 至 30 x 10(6) 精子一起冷冻在 0.5 mL 吸管中。杂交后备小母牛 (n = 865) 同步发情，并在定时 AI 或检测到发情后 12 小时进行人工授精。成年奶牛 (n = 285) 在 45 天的人工授精期内检测到发情后 12 小时进行授精。使用 FAA 阳性精子授精的女性 (n = 764) 首次接受人工授精服务的怀孕率（汇总）为 65.6%，使用 FAA 阴性精子授精的女性 (n = 386) 的怀孕率为 49.7% (P < .005)。在发情同步后备小母牛中，使用 FAA 阳性精子授精的小母牛 (n = 550) 同步人工授精服务的怀孕率为 62%，使用 FAA 阴性精子授精的小母牛 (n = 315) 的怀孕率为 45.7% (P < .005)。这些数据表明，与精子膜上不含 FAA 的公牛精液相比，使用精子膜上可检测到 FAA 的公牛精液时，自发和同步发情时首次 AI 服务的妊娠率更高。生育相关抗原是用于人工智能育种计划的公牛精子生育潜力的重要决定因素。
In this study, semen samples from 25 bulls that had passed a breeding soundness evaluation were analyzed for the presence or absence of a 31-kDa protein, known as fertility-associated antigen (FAA), on spermatozoal membranes. Eighteen bulls had FAA on sperm (FAA-positive) and seven were devoid of FAA on sperm (FAA-negative). A single ejaculate from each bull was extended and frozen with 25 to 30 x 10(6) sperm in .5-mL straws. Crossbred replacement heifers (n = 865) were estrus-synchronized and artificially inseminated either at timed AI or 12 h after they were detected in estrus. Mature cows (n = 285) were inseminated 12 h after they were detected in estrus during a 45-d AI period. Pregnancy rates (pooled) to first AI service for females (n = 764) inseminated with FAA-positive sperm were 65.6% and were 49.7% for females (n = 386) inseminated with FAA-negative sperm (P < .005). Among the estrus-synchronized replacement heifers, pregnancy rates to synchronized AI service for heifers (n = 550) inseminated with FAA-positive sperm were 62% and were 45.7% for heifers (n = 315) inseminated with FAA-negative sperm (P < .005). These data indicate that pregnancy rates to first AI service at spontaneous and synchronized estrus are higher when using semen from bulls with detectable FAA on spermatozoal membranes compared to semen from bulls devoid of FAA on membranes. Fertility-associated antigen is an important determinant for fertility potential of sperm from bulls to be used in AI breeding programs.