Chromosomal mapping of genes for transforming growth factors beta 2 and beta 3 in man and mouse: dispersion of TGF-beta gene family.
Chromosomal mapping of genes for transforming growth factors beta 2 and beta 3 in man and mouse: dispersion of TGF-beta gene family.
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发表时间：
1988
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通讯作者：
D. Barton;B. Foellmer;J. Du;J. Tamm;R. Derynck;U. Francke
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作者：
D. Barton;B. Foellmer;J. Du;J. Tamm;R. Derynck;U. Francke
两种新型转化生长因子-β，TGF-β 2和TGF-β 3的人cDNA探针，通过体细胞杂交系的Southern印迹分析，以及对于人基因座，也通过原位染色体杂交，用于在人和小鼠染色体上定位其同源基因。对于TGF-β 2，在人类1号染色体的长臂，带1 q41和小鼠1号染色体上发现了一个单一位点，最有可能在已知的保守同线区域。对于TGF-β 3，在Southern滤膜和直接染色体制备物上，杂交的主要位点在人类的14 q24。该区域与小鼠12号染色体部分同源，小鼠β 3基因座就定位在该染色体上。这些结果表明，TGF-β基因家族分布广泛，我们先前将TGF-β 1基因定位于人染色体19 q和小鼠染色体7，将α、β B和β A基因分别定位于人染色体2 q33-qter、2cen-q13和7 p15-p13。
Human cDNA probes for two new types of transforming growth factor-beta, TGF-beta 2 and TGF-beta 3, were used for mapping their cognate genes on human and mouse chromosomes by Southern blot analysis of somatic cell hybrid lines and, for the human loci, also by in situ chromosomal hybridization. For TGF-beta 2, a single site was found on the long arm of human chromosome 1, band 1q41, and on mouse chromosome 1, most likely in the known conserved syntenic region. For TGF-beta 3, the major site of hybridization, both on Southern filters and direct chromosome preparations, was at 14q24 in humans. This region is homologous in part to mouse chromosome 12, to which the murine beta 3 locus was mapped. These results indicate a wide dispersion of the TGF-beta gene family, with genes for TGF-beta 1 previously mapped by us to human chromosome 19q and mouse chromosome 7 and for inhibins alpha, beta B and beta A to human chromosomes 2q33-qter, 2cen-q13 and 7p15-p13, respectively.