Extracellular ATP mobilizes intracellular Ca2+ in T51B rat liver epithelial cells: a study involving single cell measurements.
Extracellular ATP mobilizes intracellular Ca2+ in T51B rat liver epithelial cells: a study involving single cell measurements.
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细胞外 ATP 在 T51B 大鼠肝上皮细胞中动员细胞内 Ca2：一项涉及单细胞测量的研究。
DOI：
10.1016/0014-4827(89)90198-5
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发表时间：
1989
影响因子：
3.7
通讯作者：
Berggren,PO
中科院分区：
文献类型：
--
作者：
Boynton,AL;Cooney,RV;Hill,TD;Nilsson,T;Arkhammar,P;Berggren,PO
用细胞外ATP刺激T51 B大鼠肝上皮细胞。通过fura-2在比色皿中盖玻片上的大细胞群和显微镜系统中的单细胞中测量细胞质游离Ca 2+浓度（[Ca 2 *]i）的变化。在存在和不存在细胞外Ca 2+的情况下，细胞外ATP引起[Ca 2 +] i的迅速增加，尽管在后一种情况下效果不太明显。这些发现表明，至少部分[Ca 2 +] i增加是由于细胞内结合钙的动员。用ATP刺激没有动员细胞内可释放的Ca 2+的总池，如从实验中所证明的，其中随后添加离子霉素诱发在细胞外Ca 2+不存在的情况下[Ca 2 +] i的显著增加。ATP的作用在室温下保持，但在没有连续搅拌缓冲溶液的情况下明显受损。在没有搅拌的情况下，ATP必须增加到毫摩尔范围，以引起显着的效果。单细胞测量揭示了对ATP的异质性Ca 2+响应，一些细胞未能响应[Ca 2 +]i的可检测增加。[Ca ~（2+）] i的实际增加在整个细胞质中并不均匀，但似乎开始于细胞的一个部分。即使部分[Ca ~（2+）] i的增加可能是由于ATP促进磷脂酰肌醇4，5-二磷酸的水解，从而产生InsP ~（3+）和甘油二酯所致，但在目前的条件下，DNA合成并没有启动。因此，细胞外生长因子通过激活细胞内信号通路而发挥第二信使产生的定量差异或额外的刺激作用，所述细胞内信号通路超出由[Ca 2 +] i和蛋白激酶C所代表的那些。
T51B rat liver epithelial cells were stimulated with extracellular ATP. Changes in cytoplasmic free Ca2+concentration ([Ca2*]i) were measured by fura-2 both in a large population of cells on coverslips in a cuvette and in single cells in a microscopic system. Extracellular ATP evoked a prompt increase in [Ca2+]iin both the presence and absence of extracellular Ca2+, although the effect was less pronounced in the latter case. These findings indicate that at least part of the [Ca2+]iincrease is due to mobilization of intracellularly bound calcium. Stimulation with ATP did not mobilize the total pool of intracellular releasable Ca2+, as evidenced from experiments where subsequent addition of ionomycin evoked a pronounced increase in [Ca2+]iin the absence of extracellular Ca2+. The effect of ATP was maintained at room temperature but was markedly impaired in the absence of continuous stirring of the buffer solution. In the absence of stirring, ATP had to be increased to the millimolar range in order to evoke a pronounced effect. Single cell measurements revealed a heterogenous Ca2+response to ATP, with some cells failing to respond with a detectable increase in [Ca2+]i. The actual increase in [Ca2+]iwas not uniform throughout the cytoplasm, but seemed to start in one part of the cell. Even if part of the [Ca2+]iincrease might be accounted for by ATP promoting the hydrolysis of phosphatidylinositol 4,5-bisphosphate and thereby a generation of InsP3and diacylglycerol, there was no initiation of DNA synthesis under the present conditions. Hence, extracellular growth factors exert either a quantitative difference in second messenger production or additional stimulatory effects by activating intracellular signal pathways beyond these represented by [Ca2+]iand protein kinase C.
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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通讯作者：
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影响因子：
5.5
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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