Polypeptide inhibitors of DNA synthesis and cellular proliferation produced by cultured BRL-3A rat liver cells.
Polypeptide inhibitors of DNA synthesis and cellular proliferation produced by cultured BRL-3A rat liver cells.
复制标题
由培养的 BRL-3A 大鼠肝细胞产生的 DNA 合成和细胞增殖的多肽抑制剂。
DOI：
10.1016/0014-4827(81)90337-2
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发表时间：
1981
影响因子：
3.7
通讯作者：
Straus,DS
中科院分区：
文献类型：
--
作者：
Golub,DW;Straus,DS
NIL 8仓鼠细胞系的变异体已通过一种程序进行分离，该程序选择了对增殖刺激活性（MSA，由培养的BRL-3A大鼠肝细胞产生的胰岛素样生长因子）刺激作出反应而将[3 H]胸苷掺入DNA的细胞。一些变体克隆被证明是超敏感的两个抑制剂的[3 H]胸苷掺入，存在于部分纯化的MSA制剂。已发现抑制剂是热不稳定多肽。抑制剂通过Dowex 50上的离子交换色谱与MSA共纯化，但通过Sephadex G-75上的凝胶过滤可与MSA分离。这些因子之一（IN I）已显示抑制DNA合成（通过放射自显影测定）和变异细胞的增殖。在其他几种啮齿类动物肿瘤细胞系中，测试了对INI抑制作用的敏感性，其中，Escherber H-35大鼠肝癌细胞和HSV转化的仓鼠成纤维细胞未受到该因子的抑制，而PG 19小鼠黑色素瘤细胞受到轻微抑制。
Variants of the NIL8 hamster cell line have been isolated by a procedure that selects against cells that incorporate [3H]thymidine into DNA in response to stimulation with multiplication-stimulating activity (MSA, an insulin-like growth factor produced by cultured BRL-3A rat liver cells). Some of the variant clones proved to be hypersensitive to two inhibitors of [3H]thymidine incorporation that were present in partially purified MSA preparations. The inhibitors have been found to be heat-labile polypeptides. The inhibitors copurify with MSA through ion exchange chromatography on Dowex 50, but are separable from MSA by gel filtration on Sephadex G-75. One of these factors (IN I) has been shown to inhibit DNA synthesis (as determined by autoradiography) and proliferation of the variant cells. Among several other rodent tumor cell lines tested for sensitivity to inhibition by INI, Reuber H-35 rat hepatoma cells and HSV-transformed hamster fibroblasts were not inhibited by the factor, whereas PG19 mouse melanoma cells were slightly inhibited.