A new model for nuclear lamina organization
A new model for nuclear lamina organization
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DOI：
10.1042/bst0361339
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发表时间：
2008-12-01
影响因子：
3.9
通讯作者：
Stick, Reimer
中科院分区：
文献类型：
--
作者：
Goldberg, Martin W.;Fiserova, Jindriska;Stick, Reimer
层粘连蛋白是一种中间丝蛋白，它形成一个排列在核内膜上的网络。它们为核膜提供机械强度，但似乎还有许多其他功能，如层蛋白突变引起的一系列疾病所反映的那样。与其他中间纤维蛋白不同，它们在体外不会自组装成10 nm的纤维，在体内的组织结构也不确定。我们最近重新研究了非洲爪哇卵母细胞中简单的B型叶的组织[Goldberg，Huttenlauch，Hutchison和Stick(2008)J.细胞科学]。121,215-225]，并表明它由紧密排列的8-10 nm的细丝组成，具有规则的交叉连接，与膜紧密相对。当层蛋白A在卵母细胞中表达时，它会在B层的顶部形成有组织的束状结构。这导致了一种新的叶片组织模型，本文对此进行了讨论。
Lamins are intermediate filament proteins that form a network lining the inner nuclear membrane. They provide mechanical strength to the nuclear envelope, but also appear to have many other functions as reflected in the array of diseases caused by lamin mutations. Unlike other intermediate filament proteins, they do not self-assemble into 10 nm filaments in vitro and their in vivo organization is uncertain. We have recently re-examined the organization of a simple B-type lamina in Xenopus oocytes [Goldberg, Huttenlauch, Hutchison and Stick (2008) J. Cell Sci. 121, 215-225] and shown that it consists of tightly packed 8-10 nm filaments with regular cross-connections, tightly opposed to the membrane. When lamin A is expressed in oocytes, it forms organized bundles on top of the B lamina. This has led to a new model for lamina organization which is discussed in the present paper.