Current Perspectives on Gastrointestinal Models to Assess Probiotic-Pathogen Interactions.
Current Perspectives on Gastrointestinal Models to Assess Probiotic-Pathogen Interactions.
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DOI：
10.3389/fmicb.2022.831455
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发表时间：
2022
影响因子：
5.2
通讯作者：
Forssten SD
中科院分区：
文献类型：
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作者：
Anjum M;Laitila A;Ouwehand AC;Forssten SD
有不同的模型可用于模拟人类肠上皮，因此可用于研究胃肠道中益生菌和病原体的相互作用。尽管体内模型使得研究益生菌对活体受试者的总体影响成为可能，但它们并不总是能够进行，并且普遍承诺减少动物模型的使用。因此，体外方法为研究益生菌和病原体之间的相互作用提供了更快速的工具;以及在伦理上更上级，更快，更便宜。体外模型由不太复杂的传统模型、培养板和Transwell插入物的标准2D模型以及较新的3D模型（如类器官、类肠体以及器官芯片）代表。选择的最佳模型取决于研究问题。需要适当设计的体外和/或体内研究来检查益生菌对病原体的作用机制，以获得生理学相关结果。
There are different models available that mimic the human intestinal epithelium and are thus available for studying probiotic and pathogen interactions in the gastrointestinal tract. Although, in vivo models make it possible to study the overall effects of a probiotic on a living subject, they cannot always be conducted and there is a general commitment to reduce the use of animal models. Hence, in vitro methods provide a more rapid tool for studying the interaction between probiotics and pathogens; as well as being ethically superior, faster, and less expensive. The in vitro models are represented by less complex traditional models, standard 2D models compromised of culture plates as well as Transwell inserts, and newer 3D models like organoids, enteroids, as well as organ-on-a-chip. The optimal model selected depends on the research question. Properly designed in vitro and/or in vivo studies are needed to examine the mechanism(s) of action of probiotics on pathogens to obtain physiologically relevant results.
影响因子：
2.6
作者：
Salli KM;Gürsoy UK;Söderling EM;Ouwehand AC
通讯作者：
Ouwehand AC
DOI：
10.14440/jbm.2021.347
发表时间：
2021
期刊：
Journal of biological methods
影响因子：
--
作者：
Arcidiacono S;Breedon AME;Goodson MS;Doherty LA;Lyon W;Jimenez G;Pantoja-Feliciano IG;Soares JW
通讯作者：
Soares JW