Prostate stem cell antigen predicts tumour recurrence in superficial transitional cell carcinoma of the urinary bladder
Prostate stem cell antigen predicts tumour recurrence in superficial transitional cell carcinoma of the urinary bladder
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DOI：
10.1111/j.1464-410x.2006.06153.x
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发表时间：
2006-06-01
影响因子：
4.5
通讯作者：
Kanayama, Hiro-Omi
中科院分区：
文献类型：
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作者：
Elsamman, Essam;Fukumori, Tomoharu;Kanayama, Hiro-Omi
目的探讨前列腺干细胞抗原（PSCA）在膀胱移行细胞癌（TCC）中的表达水平与临床病理分期、分级的关系;并确定PSCA mRNA表达是否预测浅表淋巴结疾病复发，膀胱移行细胞癌患者和方法实时逆转录-聚合酶链反应（RT-PCR）对97例膀胱移行细胞癌组织和36例正常膀胱尿路上皮进行了检测，并与临床病理因素进行了分析。免疫组化表达进行了检查，使用光和共聚焦免疫荧光显微镜，以验证RT-PCR data.Results 27例患者的疾病复发，而其余22例浅表膀胱移行细胞癌复发的证据。膀胱移行细胞癌组织中PSCA mRNA的表达明显高于正常尿路上皮组织（P = 0.008）。浅表性（TaT 1）肿瘤的PSCA表达显著高于肌肉浸润性（>= pT 2）肿瘤（P < 0.001）。G1-2期肿瘤患者与G3期肿瘤患者之间无显著差异（P = 0.109）。免疫组化分析显示，浅表性膀胱移行细胞癌的PSCA表达明显高于浸润性膀胱移行细胞癌。值得注意的是，从多因素分析，PSCA的表达水平是一个独立的预测疾病复发的浅表TCC（P = 0.012）。结论这些研究结果表明，PSCA表达水平测量的实时RT-PCR可能是一个有价值的预后指标浅表TCC的膀胱肿瘤复发。
Objectives To evaluate the relationship between prostate stem cell antigen (PSCA) expression level in transitional cell carcinoma (TCC) of the urinary bladder and various clinicopathological features, including stage and grade; and to determine whether PSCA mRNA expression predicts disease recurrence in superficial (not muscle-invasive) TCC of the bladder.Patients and Methods Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed on 97 TCC tissue samples and in 36 samples of normal bladder urothelium; the findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using light and confocal immunofluorescence microscopy to validate the RT-PCR data.Results Twenty-seven patients developed disease recurrence, while the remaining 22 had no evidence of recurrence of superficial TCC of the bladder. There was significantly higher PSCA mRNA expression in TCC than in normal urothelium samples (P = 0.008). Superficial (TaT1) tumours had significantly higher PSCA expression than muscle-invasive (>= pT2) tumours (P < 0.001). There was no significant difference between patients with G1-2 tumours and those with G3 tumours (P = 0.109). Immunohistochemical analysis showed markedly greater PSCA expression in superficial than invasive TCC. Notably, from a multivariate analysis, the expression level of PSCA was an independent predictor of disease recurrence in superficial TCC (P = 0.012).Conclusions These findings suggest that the PSCA expression level measured by real-time RT-PCR could be a valuable prognostic marker for tumour recurrence in superficial TCC of the bladder.