Triolein-cholesteryl oleate-cholesterol-lecithin emulsions: structural models of triglyceride-rich lipoproteins.
Triolein-cholesteryl oleate-cholesterol-lecithin emulsions: structural models of triglyceride-rich lipoproteins.
复制标题
三油酸甘油酯-胆固醇油酸酯-胆固醇-卵磷脂乳液：富含甘油三酯的脂蛋白的结构模型。
DOI：
10.1021/bi00271a030
复制
发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Small,DM
中科院分区：
文献类型：
--
作者：
Miller,KW;Small,DM
Kurt W. Miller*和Donald M. Small*摘要：研究了由三油酸酯（TO）、油酸胆固醇（CO）、胆固醇(C)和蛋黄磷脂酰胆碱(L)组成的乳状液中脂质的组织。在一系列乳剂中用CO代替TO，得到与富甘油三酯脂蛋白亚组分中甘油三酯：胆固醇酯比率相当的TO: CO比率。表面相中TO的质量分数（0.02 ~ 0.05）与乳剂中TO的含量无关。然而，表面相中CO的重量分数取决于乳液中CO的百分比，即使乳液中CO的含量为13.7%，其重量分数也< 0.004。当用enco代替TO时，能够分散乳糜微粒的总颗粒C （Fraser, 1970; Yokoyama & Zilversmit, 1965； Lossow等人，1969）和极低密度脂蛋白（VLDL） 1 （Sata等人，1972；Bsenberg等人，1973）所占的百分比可以按大小分成化学上不均匀的亚组分。亚组分中脂蛋白的化学组成与其颗粒直径有关。与小脂蛋白相比，大脂蛋白含有较多的非极性脂质、甘油三酯和胆固醇酯，而较少的极性脂质、胆固醇(C)和磷脂。VLDL亚组分内的甘油三酯：胆固醇酯比率是大小依赖性的；比率在
Kurt W. Miller* and Donald M. Small* abstract: The organization of lipids within emulsions com-posed of triolein (TO), cholesteryl oleate (CO), cholesterol (C), and egg yolk phosphatidylcholine (L) was examined. CO was substituted for TO in a series of emulsions to obtain TO: CO ratios comparable to thetriglyceride: cholesterol ester ratios observed in subfractions of triglyceride-rich lipoproteins. The weight fraction of TO in the surface phase (0.02-0.05) was independent of the TO content of the emulsions. However, the weight fraction of CO in the surface phase depended upon the percentage of CO in the emulsions and was< 0.004 even when 13.7% CO was present in the emulsion. WhenCO was substituted for TO, the percent of the total particle C whichI^ lydisperse chylomicrons (Fraser, 1970; Yokoyama & Zilversmit, 1965; Lossow et al., 1969) and very low density lipoproteins (VLDL) 1 (Sata et al., 1972; Bsenberg et al., 1973) can be fractionated by size into chemically heterogeneous subfractions. The chemical composition of lipoproteins within the subfractions is related to their particlediameters. Large lipoproteins contain a greater percentage of nonpolar lipids, triglyceride and cholesterol ester, and smaller percentages of polar lipids, cholesterol (C) and phospholipid, than small li-poproteins. The triglyceride: cholesterol ester ratios within VLDL subfractions are size dependent; the ratios decrease in