Electrochemical analysis of 8-hydroxy-2'-deoxyguanosine with enhanced sensitivity based on exonuclease-mediated functional nucleic acid
Electrochemical analysis of 8-hydroxy-2'-deoxyguanosine with enhanced sensitivity based on exonuclease-mediated functional nucleic acid
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DOI：
10.1016/j.talanta.2019.02.080
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Li Genxi
中科院分区：
文献类型：
--
作者：
Zheng Ji;Gao Tao;Shi Hai;Huang Yue;Xiang Yang;Li Genxi
本文提出了一种基于外切酶介导的功能核酸的电化学方法，用于灵敏分析8-羟基-2′-脱氧鸟苷，这是一种广泛用于研究氧化损伤相关疾病的关键生物标志物。在设计中，外切酶不仅可以区分目标的存在，还可以抑制背景噪声，从而提高灵敏度。此外，在功能核酸中设计DNAzyme可以进一步提高信号产生过程中分析的灵敏度。因此，外切酶介导的功能性核酸可以确保检测的高灵敏度。进一步研究表明，8-羟基-2′-脱氧鸟苷在0.01 nM ~ 7.0 μM范围内具有良好的线性关系，检出限为6.82 pM。新方法已成功应用于尿液中8-OHdG的测定，结果良好，具有较大的实际应用潜力。
In this work, an electrochemical method for sensitive analysis of 8-hydroxy-2′-deoxyguanosine, a key biomarker that is widely used to study oxidative injury-related diseases, is proposed based on exonuclease-mediated functional nucleic acid. In the design, exonuclease can not only distinguish the existence of target, but also suppress the background noise, thus the sensitivity can be enhanced. Moreover, DNAzyme designed in the functional nucleic acid can further improve the sensitivity of the analysis during signal generation process. Therefore, exonuclease-mediated functional nucleic acid may ensure high sensitivity of the assay. Further studies reveal that the detection of 8-hydroxy-2′-deoxyguanosine can be achieved with a linearity from 0.01 nM to 7.0 μM and a detection limit of 6.82 pM. The new method has also been successfully applied to the determination of 8-OHdG in urine with good results, indicating its great potential for practical use.