Hepatitis B virus contains pre-S gene-encoded domains
Hepatitis B virus contains pre-S gene-encoded domains
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乙型肝炎病毒含有前S基因编码结构域
DOI：
10.1038/315154a0
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发表时间：
1985
期刊：
影响因子：
64.8
通讯作者：
C. E. Stevens
中科院分区：
文献类型：
--
作者：
A. Neurath;Stephen B.H. Kent;N. Strick;P. Taylor;C. E. Stevens
B型肝炎病毒（HBV）DNA上的开放阅读框之一包含病毒包膜蛋白的编码区（称为env基因）。对信使RNA转录的研究表明，该基因有可能编码三种相关蛋白1：（1）一种226个氨基酸的蛋白，被鉴定为HBV包膜的主要蛋白组分，称为S-蛋白;（2）一种在N-末端具有55个额外氨基酸的蛋白，由S-基因上游的env基因的一部分编码（前S）;（3）对应于完整env基因的蛋白质（pre-S + S）。来自蛋白质（2）和（3）N端的合成肽及其抗血清已被用于研究前S序列的存在和性质。这里给出的结果提供了所有三种env编码的蛋白质存在于HBV颗粒中的明确证据;对应于编码pre-S的基因的合成肽具有高度免疫原性，并且可以用于诊断试验，以检测优先识别HBV的人血清中的抗体;这种特异于前S决定簇的抗体在B型肝炎感染期间和通过用HBV蛋白免疫而被引发（2）和（3）;美国许可的B型肝炎疫苗不含前S蛋白; HBV包膜的前S蛋白含有肝细胞特异性识别的结构域。这些发现表明，前S决定簇在病毒中和反应中很重要，应该存在于HBV疫苗中。
One of the open reading frames on hepatitis B virus (HBV) DNA comprises the coding region (designated the env gene) for the virus envelope proteins. Studies on messenger RNA transcription suggest that this gene has the potential to code for three related proteins1: (1) a protein of 226 amino acids identified as a major protein constituent of the HBV envelope, termed S-protein; (2) a protein with 55 additional amino acids at the N-terminal coded for by a portion of the env gene upstream of the S-gene (pre-S); (3) a protein corresponding to the entire env gene (pre-S + S). Synthetic peptides from the N-termnals of proteins (2) and (3), and antisera to them have been used to study the occurrence and properties of pre-S sequences. The results presented here provide unambiguous evidence that all three env encoded proteins are present in HBV particles; synthetic peptides corresponding to the gene encoding pre-S are highly immunogenic and can be used in diagnostic tests for detection in human sera of antibodies preferentially recognizing HBV; such antibodies, specific for pre-S determinants, are elicited during hepatitis B infection and by immunization with HBV proteins (2) and (3); the hepatitis B vaccine licensed in the United States does not contain pre-S proteins; and the pre-S proteins of the HBV envelope contain domains specifically recognized by liver cells. These findings suggest that pre-S determinants are important in virus-neutralizing responses and should be present in HBV vaccines.