EPISTATIC GENE INTERACTIONS IN THE CONTROL OF DIVISION IN FISSION YEAST
EPISTATIC GENE INTERACTIONS IN THE CONTROL OF DIVISION IN FISSION YEAST
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DOI：
10.1038/279428a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
FANTES, P
中科院分区：
文献类型：
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作者：
FANTES, P
目前人们对控制细胞分裂时间的机制很感兴趣。已经发现对照的某些特征对于多种真核生物是共同的。特别是，细胞大小作为影响细胞周期进程的参数的重要性已被报道用于哺乳动物细胞1，2和几种单细胞真核生物3 -6。几个系统的另一个共同特征是生长条件对分裂周期事件的时间和细胞大小有直接影响7 -9。在裂殖酵母裂殖酵母中，细胞大小6和营养条件9都被证明会影响循环动力学。生物体已被广泛用作真核系统的模型，主要是因为易于测量细胞大小，因为分裂是通过二元分裂发生的。最近，它的遗传易处理性导致了细胞分裂周期（cdc）突变体的分离12，以及在控制与分裂周期协调生长中改变的weemutants 13 -15。这种控制突变体的存在允许一个更直接的方法来调查的分子基础的分裂控制，在其他systems 4，16- 18中使用的间接方法相反。粟酒Weemutants最显著的特性是它们减小的细胞大小13，14。对这些突变体的分析15，19和其他证据9表明，对细胞分裂时间的控制通常在进入有丝分裂时起作用。由于许多cdc基因的功能是有丝分裂所特别需要的，因此可以预期wee和cdc突变体之间的相互作用会影响有丝分裂。我在这里报告，由缺陷的dc 25等位基因引起的有丝分裂缺陷在突变体中被抑制。wee 1突变体几乎完全抑制，而wee2.1突变是一个不太有效的抑制剂。这些发现的意义被认为是控制有丝分裂的遗传模型。
THERE is currently much interest in the mechanism which controls the timing of cell division. Certain features of the control have been found to be common to a variety of eukaryotes. In particular, the importance of cell size as a parameter affecting cell cycle progress has been reported for mammalian cells1,2and for several single-celled eukaryotes3–6. Another feature common to several systems is that growth conditions have a direct effect on the timing of division cycle events7–9, and on cell size9,10. In the fission yeastSchizosaccharomyces pombe, both cell size6and nutritional conditions9have been shown to affect cycle kinetics. The organism has been used extensively as a model eukaryotic system, largely because of the ease of measuring cell size and because division occurs by binary fission11. More recently, its genetic tractability has led to the isolation of cell division cycle (cdc) mutants12, and also ofweemutants altered in the control coordinating growth with the division cycle13–15. The existence of such control mutants allows a more direct approach to the investigation of the molecular basis of division control, in contrast to the indirect methods used in other systems4,16–18.weemutants are so far unique toS. pombe. The most conspicuous property ofweemutants is their reduced cell size13,14. Analysis of these mutants15,19and other evidence9has shown that control over cell division timing normally acts at entry to mitosis. As the function of a number ofcdcgenes is specifically required for mitosis12, interactions betweenweeandcdcmutants which affect mitosis might be expected. I report here that the mitotic defect caused by a defectivecdc25 allele is suppressed inweemutants. Suppression bywee1 mutants is almost complete, while thewee2.1 mutation is a less effective suppressor. The significance of these findings for genetic models of the control of mitosis is considered.