Developmental lead acetate exposure induces embryonic toxicity and memory deficit in adult zebrafish
Developmental lead acetate exposure induces embryonic toxicity and memory deficit in adult zebrafish
复制标题
发育过程中醋酸铅暴露会导致成年斑马鱼胚胎毒性和记忆缺陷
DOI：
10.1016/j.ntt.2012.09.001
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发表时间：
2012-11-01
影响因子：
2.9
通讯作者：
Huang, Changjiang
中科院分区：
文献类型：
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作者：
Chen, Jiangfei;Chen, Yuanhong;Huang, Changjiang
铅是一种持久性金属，普遍存在于我们的生活环境中。本研究旨在研究铅对斑马鱼的胚胎毒性、行为反应和成年学习记忆障碍。受精后120h，醋酸铅可致小鼠出现鱼囊不充气、脊柱弯曲、卵黄囊肿胀等畸形，EC50值为0.29 mg/L。暴露于PBAC后，斑马鱼胚胎的自发运动显著改变，其特征是尾巴弯曲频率。行为学评估表明，铅暴露改变了斑马鱼幼体的行为反应，在从6 HPF到96 HPF的PBAC暴露的第一分钟内，斑马鱼幼鱼的行为反应被检测到过度活动，并且在铅暴露后，在黑暗中和在120 HPF的光照下，游泳速度出现了不同的剂量依赖性变化。学习/记忆任务分析显示，暴露于6-120 HPF的PBAC的胚胎在成年期出现学习/记忆缺陷，表现为寻找食物的准确率显着下降，寻找时间显着增加。总体而言，我们的结果表明，低剂量的发育铅暴露导致斑马鱼胚胎毒性、行为改变和成年学习/记忆障碍。(C)2012 Elsevier Inc.保留所有权利。
Lead is a persistent metal and commonly present in our living environment. The present study was aimed to investigate lead-induced embryonic toxicity, behavioral responses, and adult learning/memory deficit in zebrafish. Lead acetate (PbAc) induced malformations such as uninflated swim bladder, bent spine and yolk-sac edema with an EC50 of 0.29 mg/L at 120 h post fertilization (hpf). Spontaneous movement as characterized by tail bend frequency was significantly altered in zebrafish embryos following exposure to PbAc. Behavior assessment demonstrated that lead exposure changed behavioral responses in zebrafish larvae, as hyperactivity was detected within the first minute of light-to-dark transition in the fish exposed to PbAc from 6 to 96 hpf, and a different dose-dependent change was found in swimming speeds in the dark and in the light at 120 hpf following lead exposure. Learning/memory task assay showed that embryos exposed to PbAc from 6 to 120 hpf developed learning/memory deficit at adulthood as exhibited by a significant decrease in accuracy rate to find the food and a significant increase in finding time. Overall, our results suggested that low dose of developmental lead exposure resulted in embryonic toxicity, behavioral alteration, and adult learning/memory deficit in zebrafish. (C) 2012 Elsevier Inc. All rights reserved.