Identification of X-linked quantitative trait loci affecting cold tolerance in Drosophila melanogaster and fine mapping by selective sweep analysis.
Identification of X-linked quantitative trait loci affecting cold tolerance in Drosophila melanogaster and fine mapping by selective sweep analysis.
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DOI：
10.1111/j.1365-294x.2010.04951.x
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发表时间：
2011-02
影响因子：
4.9
通讯作者：
Stephan W
中科院分区：
文献类型：
--
作者：
Svetec N;Werzner A;Wilches R;Pavlidis P;Alvarez-Castro JM;Broman KW;Metzler D;Stephan W
黑腹果蝇是一种世界性物种，栖息于多种环境中。耐冷性是黑腹果蝇不同群体中自然分离遗传变异的一个性状。先前的工作已发现数量性状位点（QTL）仅位于第二和第三染色体上。为了深入了解 X 染色体上耐冷性的遗传结构，并将结果与我们的选择性扫描分析进行比较，绘图群体源自仅 X 染色体起源不同的替代品系之间的杂交：一个源自欧洲近交系，另一个源自非洲近交系。我们在黑腹果蝇X染色体上共发现了6个耐冷因子QTL。尽管复合区间作图显示性别之间的 QTL 谱略有不同，但一致的模型表明大多数 QTL 在性别之间重叠，并且每个 QTL 解释了大约 5-14% 的遗传方差（可能稍微高估了）。等位基因效应很大程度上是累加性的，但我们也检测到了两种显着的相互作用。总而言之，这为多个 QTL 提供了证据，这些 QTL 沿着整个 X 染色体传播，其影响范围从低到中。一种检测到的越界 QTL 以不同的方式影响两性的耐寒性。虽然雌性受益于欧洲等位基因，提高了其耐寒性，但雄性往往在非洲等位基因方面表现更好。最后，利用选择性扫描作图，鉴定了与QTL共定位的耐冷候选基因CG16700。
Drosophila melanogaster is a cosmopolitan species that colonizes a great variety of environments. One trait that shows abundant evidence for naturally segregating genetic variance in different populations of D. melanogaster is cold tolerance. Previous work has found quantitative trait loci (QTL) exclusively on the second and the third chromosomes. To gain insight into the genetic architecture of cold tolerance on the X chromosome and to compare the results with our analyses of selective sweeps, a mapping population was derived from a cross between substitution lines that solely differed in the origin of their X chromosome: one originates from a European inbred line and the other one from an African inbred line. We found a total of six QTL for cold tolerance factors on the X chromosome of D. melanogaster. Although the composite interval mapping revealed slightly different QTL profiles between sexes, a coherent model suggests that most QTL overlapped between sexes, and each explained around 5–14% of the genetic variance (which may be slightly overestimated). The allelic effects were largely additive, but we also detected two significant interactions. Taken together, this provides evidence for multiple QTL that are spread along the entire X chromosome and whose effects range from low to intermediate. One detected transgressive QTL influences cold tolerance in different ways for the two sexes. While females benefit from the European allele increasing their cold tolerance, males tend to do better with the African allele. Finally, using selective sweep mapping, the candidate gene CG16700 for cold tolerance colocalizing with a QTL was identified.