Simultaneous analysis of prostaglandin glyceryl esters and prostaglandins by electrospray tandem mass spectrometry
Simultaneous analysis of prostaglandin glyceryl esters and prostaglandins by electrospray tandem mass spectrometry
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DOI：
10.1016/j.ab.2005.05.005
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发表时间：
2005-08-15
影响因子：
2.9
通讯作者：
Marnett, LJ
中科院分区：
文献类型：
--
作者：
Kingsley, PJ;Rouzer, CA;Marnett, LJ
建立了一种新发现的类花生酸前列腺素甘油酯（PG-Gs）的高效液相色谱（HPLC）正离子电喷雾串联质谱定量分析方法。同时定量所有四种PG-G（PGE（2）-G、PGD（2）-G、PGF（2 α）-G和6-酮基-PGF（1 α）-G）和通过其水解形成的洋地黄素（PGs）。通过反相固相萃取纯化分析物，通过反相HPLC分离，并使用选择的反应监测在三重四极杆质谱仪上定量。通过使用五氘代（PG-Gs）或四氘代（PGs）类似物的稳定同位素稀释实现定量。对于四种不同浓度水平的所有分析物，细胞培养基中的分析物回收率>43%。每种分析物的定量限在25 fmol柱上范围内，分析物在约500倍范围内显示线性响应。该方法允许同时分析几种PG-G和PG，而无需多步样品纯化或衍生化。(C)2005年爱思唯尔公司All rights reserved.
A high-performance liquid chromatography (HPLC) positive-ion electrospray ionization tandem mass spectrometry method for the quantification of prostaglandin glyceryl esters (PG-Gs), a newly discovered class of eicosanoids, is described. All four PG-Gs (PGE(2)-G, PGD(2)-G, PGF(2 alpha)-G, and 6-keto-PGF(1 alpha)-G) and the prostaglandins (PGs) that are formed by their hydrolysis are simultaneously quantified. Analytes were purified via reverse-phase solid-phase extraction, separated by reverse-phase HPLC, and quantified on a triple-quadrupole mass spectrometer using selected reaction monitoring. Quantification was achieved by stable isotope dilution employing penta-deuterated (PG-Gs) or tetra-deuterated (PGs) analogs. Analyte recovery from cell culture medium was >43% for all analytes at four different concentration levels. The limit of quantification is in the range of 25 fmol on-column for each analyte and the analytes exhibit a linear response over approximately a 500-fold range. This method allows simultaneous profiling of several PG-Gs and PGs without multistep sample purification or derivatization. (C) 2005 Elsevier Inc. All rights reserved.