A direct solid sampling analysis method for the detection of silver nanoparticles in biological matrices
A direct solid sampling analysis method for the detection of silver nanoparticles in biological matrices
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DOI：
10.1007/s00216-015-9108-1
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发表时间：
2016-01-01
影响因子：
4.3
通讯作者：
Leopold, Kerstin
中科院分区：
文献类型：
--
作者：
Feichtmeier, Nadine S.;Ruchter, Nadine;Leopold, Kerstin
工程银纳米颗粒（AgNPs）由于其强大的抗菌性能而应用于食品接触材料，因此可能进入人类食物链。因此，需要开发简单、灵敏和快速的分析筛选方法来测定复杂生物基质中的AgNPs。本研究采用固体进样高分辨连续源石墨炉原子吸收光谱法（GFAAS）。最近报道的一种新的评估策略使用各自的GFAAS信号的雾化延迟作为AgNP的重要指标，从而允许在样品中区分AgNP和离子银（Ag+），而无需精心的样品预处理。这种方法被进一步开发并应用于各种生物样品。其适用性通过研究掺入离子银或AgNPs的八种不同的食物样品（欧芹、苹果、胡椒、奶酪、洋葱、意大利面、玉米粉和小麦粉）而被批准。此外，银浸渍聚丙烯食品储存盒的银纳米粒子的迁移到新鲜的辣椒进行了观察和贻贝样品从实验室暴露研究与银进行了调查。银离子和20-nm AgNP之间的雾化延迟（Δ t（ad））的差异在从玉米粉的-2.01 +/-1.38秒到贻贝组织的+2.06 +/-1.08秒的范围内变化。然而，在所有研究的基质中差异都是显著的，因此表明存在/不存在AgNP。此外，模型基质（纤维素，明胶和水）的调查给出了第一个矩阵依赖性趋势的迹象。重现性和均匀性试验证实了该方法的适用性。
Engineered silver nanoparticles (AgNPs) are implemented in food contact materials due to their powerful antimicrobial properties and so may enter the human food chain. Hence, it is desirable to develop easy, sensitive and fast analytical screening methods for the determination of AgNPs in complex biological matrices. This study describes such a method using solid sampling high-resolution continuum source graphite furnace atomic absorption spectrometry (GFAAS). A recently reported novel evaluation strategy uses the atomization delay of the respective GFAAS signal as significant indicator for AgNPs and thereby allows discrimination of AgNPs from ionic silver (Ag+) in the samples without elaborate sample pre-treatment. This approach was further developed and applied to a variety of biological samples. Its suitability was approved by investigation of eight different food samples (parsley, apple, pepper, cheese, onion, pasta, maize meal and wheat flour) spiked with ionic silver or AgNPs. Furthermore, the migration of AgNPs from silver-impregnated polypropylene food storage boxes to fresh pepper was observed and a mussel sample obtained from a laboratory exposure study with silver was investigated. The differences in the atomization delays (Delta t(ad)) between silver ions and 20-nm AgNPs vary in a range from -2.01 +/- 1.38 s for maize meal to + 2.06 +/- 1.08 s for mussel tissue. However, the differences were significant in all investigated matrices and so indicative of the presence/absence of AgNPs. Moreover, investigation of model matrices (cellulose, gelatine and water) gives the first indication of matrix-dependent trends. Reproducibility and homogeneity tests confirm the applicability of the method.