Retro-orbital injection is an effective route for radiopharmaceutical administration in mice during small-animal PET studies
Retro-orbital injection is an effective route for radiopharmaceutical administration in mice during small-animal PET studies
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DOI：
10.1097/mnm.0b013e3281fbd42b
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发表时间：
2007-07-01
影响因子：
1.5
通讯作者：
Fanti, Stefano
中科院分区：
文献类型：
--
作者：
Nanni, Cristina;Pettinato, Cinzia;Fanti, Stefano
背景和目的小动物PET在临床前研究中越来越重要。在啮齿类动物中，放射性示踪剂通常通过尾静脉给药。这个过程可能非常困难和耗时，因为软组织外渗非常频繁，并且尾部疤痕可以防止在最初失败后重复注射。我们的研究的目的是比较眶后（RO）与尾静脉注射0.0的F-18-FDG和C-11-胆碱在小鼠小动物PETstudy.Methods的管理，我们评估了四个健康的雌性ICR CD 1小鼠根据以下协议。第1天：每只动物静脉注射28 M Bq 11 C-胆碱。10分钟和40分钟后进行PET扫描。第2天：每只动物接受28 M Bq 11 C-胆碱的RO注射。10 min和40 min后进行PET扫描。第3天：每只动物接受LV。注射28 MBq的18F-FDG。在60分钟和120分钟后进行PET扫描。第4天：每只动物接受28 MBq F-18-FDG的RO注射。在60分钟和120分钟后进行PET扫描。在气体麻醉下进行给药和图像采集。对于FDG研究，动物禁食2小时，并在注射后保持睡眠20-30分钟，以避免肌肉摄取。用二维OSEM重建图像。对于每次扫描，在肝脏、肾脏、肺、脑、心脏棕色脂肪和肌肉上绘制ROI，并计算SUV。最后，我们比较了胆碱静脉标准采集与胆碱RO标准采集、胆碱静脉延迟采集与胆碱RO延迟采集、FDG静脉标准采集与FDG RO标准采集，结果RO注射F-18-FDG和C-11-胆碱与静脉注射18 F-18-FDG相比，FDG的标准和延迟的acquisition.Conclusion RO管理在小鼠静脉给药是一个更容易和更快的过程中的技术优势。然而，它的使用需要高的比活性，而其在肽和其他受体特异性放射性药物中的价值需要进一步评估。
Background and aim Small-animal PET is acquiring importance for pre-clinical studies. In rodents, radiotracers are usually administrated via the tail vein. This procedure can be very difficult and time-consuming as soft tissue extravasations are very frequent and tail scars can prevent repeated injections after initial failure. The aim of our study was to compare the retro-orbital (RO) versus tail vein intravenous 0.0 administration of F-18-FDG and C-11-choline in mice for small-animal PET studies.Methods We evaluated four healthy female ICR CD1 mice according to the following protocol. Day 1: each animal underwent an i.v. injection of 28 M Bq of 11 C-choline. PET scan was performed after 10 min and 40 min. Day 2: each animal received an RO injection of 28 M Bq of 11 C-choline. A PET scan was performed after 10 min and 40 min. Day 3: each animal received an Lv. injection of 28 M Bq of 18F-FDG. A PET scan was performed after 60 min and 120 min. Day 4: each animal received an RO injection of 28 MBq of F-18-FDG. A PET scan was performed after 60 min and 120 min. Administration and image acquisition were performed under gas anaesthesia. For FDG studies the animals fasted for 2 h and were kept asleep for 20-30 min after injection, to avoid muscular uptake. Images were reconstructed with 2-D OSEM. For each scan ROIs were drawn on liver, kidneys, lung, brain, heart brown fat and muscles, and the SUV was calculated. We finally compared choline i.v. standard acquisition to choline RO standard acquisition; choline i.v. delayed acquisition to choline RO delayed acquisition; FDG i.v. standard acquisition to FDG RO standard acquisition; FDG i.v. delayed acquisition to FDG RO delayed acquisition.Results The RO injections for both F-18-FDG and C-11-choline were comparable to the intravenous injection of 18F-FDG for the standard and delayed acquisitions.Conclusion The RO administration in mice represents a technical advantage over intravenous administration in being an easier and faster procedure. However, its use requires high specific activity while its value in peptides and other receptor-specific radiopharmaceuticals needs further assessment.