Administration of poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) as adjuvant activated mixed Th1/Th2 immune responses in pigs
Administration of poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) as adjuvant activated mixed Th1/Th2 immune responses in pigs
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DOI：
10.1016/j.vetimm.2012.01.021
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发表时间：
2012-05-15
影响因子：
1.8
通讯作者：
Mutwiri, George K.
中科院分区：
文献类型：
--
作者：
Dar, Arshud;Lai, Ken;Mutwiri, George K.
选择最佳佐剂以增强抗原特异性免疫应答的效力和寿命对于开发更有效和更安全的疫苗一直是必要的。一种称为聚磷腈（PCEP）的新佐剂的平衡型免疫增强能力已在小鼠中得到证实。在本研究中，我们比较了对含有胸膜肺炎放线杆菌外膜抗原（OmlA）与PCEP或Emulsigen（R）作为佐剂的疫苗制剂的体液和细胞免疫应答。我们的数据显示，当OmlA抗原与PCEP和CpG ODN一起配制时，免疫应答显著改善并向更平衡的免疫应答转变。此外，与Emulsigen（R）相反，用PCEP免疫不会导致局部注射部位反应，突出了其作为猪安全有效佐剂的潜力。此外，PCEP的配制、给药的容易性和相对低的每剂量成本使其成为猪的有希望的佐剂。(C)2012爱思唯尔有限公司版权所有。
The selection of an optimal adjuvant to enhance the potency and longevity of antigen specific immune responses has always been imperative for the development of more effective and safer vaccines. A balanced type of immune enhancing ability of a new adjuvant known as polyphosphazene (PCEP) has been demonstrated in mice. In the present study we have compared the humoral and cellular immune responses to vaccine formulations containing Actinobacillus pleuropneumoniae outer membrane antigen (OmlA) with PCEP or Emulsigen (R) as adjuvants. Our data showed a significant improvement and a shift toward more balanced immune responses when OmlA antigen was formulated with PCEP and CpG ODN. Moreover, in contrast to Emulsigen (R), immunization with PCEP did not result in local injection site reactions highlighting its potential as a safe and effective adjuvant for pigs. Further, the ease of formulation, administration and relatively low per dose cost of PCEP make it a promising adjuvant for pigs. (C) 2012 Elsevier B.V. All rights reserved.