Phosphotyrosine Picked Threonine Kinase stimulates the proliferation of human osteosarcoma cells in vitro and in vivo(TTK promotes osteosarcoma proliferation)
Phosphotyrosine Picked Threonine Kinase stimulates the proliferation of human osteosarcoma cells in vitro and in vivo(TTK promotes osteosarcoma proliferation)
复制标题
磷酸酪氨酸挑选的苏氨酸激酶在体外和体内刺激人骨肉瘤细胞增殖（TTK促进骨肉瘤增殖）
作者：
Zhe-Xiang Wang;Shao-Chun Ren;Jing Ren
骨肉瘤是最常见的原发性骨肿瘤，主要发病人群为青少年。单纯手术治疗骨肉瘤的存活率为10%-20%。与其他肿瘤相比，骨肉瘤的基础研究较少，我们需要更多的机制来提高生存率。TTK已被广泛报道为多种类型癌症的致癌基因，它也被称为临床治疗靶点。本研究旨在评估TTK在人骨肉瘤组织中的表达水平及其与骨肉瘤患者临床特征的联系，并评估其在骨肉瘤发展中的潜在作用。方法：采用免疫组化（IHC）法检测TTK在74例骨肉瘤组织及其邻近组织中的表达水平。根据TTK在肿瘤组织中的染色强度将患者分为TTK高表达组和TTK低表达组。分析TTK表达水平与临床特征之间可能存在的相关性，并通过集落形成和CCK8检测TTK对骨肉瘤细胞增殖的影响。采用动物模型检测TTK对肿瘤生长的影响。结果：TTK在人骨肉瘤组织中异常高表达。TTK与骨肉瘤患者肿瘤大小（P=0.004*）、临床分期（P=0.014*）等临床特征有明显相关性。我们的研究结果还表明，抑制TTK显著抑制骨肉瘤细胞的体外增殖，阻断肿瘤生长。在老鼠身上。结论：我们证明TTK参与骨肉瘤的发展，因此我们建议TTK应该被认为是一个有希望的骨肉瘤治疗靶点。
Introduction: Osteosarcoma is the most common primary bone tumor, and the main affected population is adolescents. The survival of osteosarcoma was 10%-20% when surgery as a single treatment. Basic research on osteosarcoma is less than other tumors, we need more mechanisms to improve the survival rate. TTK has been widely reported as an oncogene in multiple types of cancers, and it is also known as a clinical therapeutic target. This study aims to assess TTK expression levels in human osteosarcoma tissues and its link with the clinical characteristics of osteosarcoma patients, and to evaluate the potential role in osteosarcoma development. Methods: Immunohistochemical (IHC) assays were conducted to detect the expression levels of TTK in a total number of 74 osteosarcoma tissues and the corresponding adjacent tissues. Furthermore, according to the staining intensity of TTK in tumor tissues, patients were divided into TTK high and low expression groups. The possible correlation between TTK expression levels and clinical features were analyzed, and the effects of TTK on osteosarcoma cell proliferation was detected through colony formation and CCK8 assays. The effects of TTK on tumor growth was detected using an animal model. Results: TTK was abnormal highly expressed in human osteosarcoma tissues. Meanwhile, TTK was obviously correlated to the clinical characteristics such as tumor size (P=0.004*) and clinical stage (P=0.014*) of osteosarcoma patients. Our results also revealed that the inhibition of TTK dramatically suppressed the proliferation of osteosarcoma cells in vitro and blocked tumor growth.in mice. Conclusions: We demonstrated the involvement of TTK in the development of osteosarcoma, and therefore we suggested that TTK should be considered as a promising therapy target for osteosarcoma.