Structural evolution of CatSper1 in rodents is influenced by sperm competition, with effects on sperm swimming velocity.
Structural evolution of CatSper1 in rodents is influenced by sperm competition, with effects on sperm swimming velocity.
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DOI：
10.1186/1471-2148-14-106
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发表时间：
2014-05-16
影响因子：
3.4
通讯作者：
Roldan ER
中科院分区：
文献类型：
--
作者：
Vicens A;Tourmente M;Roldan ER
来自竞争雄性的精子之间为成功受精而进行的竞争（即，精子竞争）是驱动雄性生殖性状进化和促进生殖功能相关基因正选择的重要选择力量。正选择已被确定在生殖蛋白中，在核苷酸水平上显示快速分化。其他突变，如插入和缺失（indels），也发生在蛋白质编码序列中。这些结构变化存在于生殖基因中，并导致编码蛋白的长度变化，也可能受到正选择和精子竞争的影响。Catsper 1是一个生殖基因，编码一个生殖细胞系特异性电压门控钙通道，对精子运动和受精至关重要。正向选择似乎促进了哺乳动物物种中CatSper 1 N-末端区域的indel固定。然而，目前尚不清楚这些变化背后的选择力及其对精子功能的影响。我们测试了CatSper 1的N-末端区域的长度变化是否受到一组密切相关的啮齿动物鼠科亚科物种中精子竞争强度的影响。我们的研究结果显示，CatSper 1的序列长度和相对睾丸质量，精子竞争水平的一个很好的代理之间的负相关。由于CatSper 1是重要的精子鞭毛运动，我们研究了如果长度变化的N-末端的CatSper 1是链接到精子游泳速度的变化。我们发现CatSper 1长度和几个精子速度参数之间呈负相关。总而言之，我们的研究结果表明，精子竞争选择缩短CatSper 1的细胞内区域，这反过来又提高了精子游泳速度，这是受精成功的一个重要和适应性特征。
Competition between spermatozoa from rival males for success in fertilization (i.e., sperm competition) is an important selective force driving the evolution of male reproductive traits and promoting positive selection in genes related to reproductive function. Positive selection has been identified in reproductive proteins showing rapid divergence at nucleotide level. Other mutations, such as insertions and deletions (indels), also occur in protein-coding sequences. These structural changes, which exist in reproductive genes and result in length variation in coded proteins, could also be subjected to positive selection and be under the influence of sperm competition. Catsper1 is one such reproductive gene coding for a germ-line specific voltage-gated calcium channel essential for sperm motility and fertilization. Positive selection appears to promote fixation of indels in the N-terminal region of CatSper1 in mammalian species. However, it is not known which selective forces underlie these changes and their implications for sperm function. We tested if length variation in the N-terminal region of CatSper1 is influenced by sperm competition intensity in a group of closely related rodent species of the subfamily Murinae. Our results revealed a negative correlation between sequence length of CatSper1 and relative testes mass, a very good proxy of sperm competition levels. Since CatSper1 is important for sperm flagellar motility, we examined if length variation in the N-terminus of CatSper1 is linked to changes in sperm swimming velocity. We found a negative correlation between CatSper1 length and several sperm velocity parameters. Altogether, our results suggest that sperm competition selects for a shortening of the intracellular region of CatSper1 which, in turn, enhances sperm swimming velocity, an essential and adaptive trait for fertilization success.
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影响因子：
4.8
作者：
Chanchevalap, S;Yang, ZJ;Jiang, C
通讯作者：
Jiang, C
DOI：
10.1073/pnas.0809990106
发表时间：
2009-01-27
影响因子：
11.1
作者：
Fitzpatrick, John L.;Montgomerie, Robert;Balshine, Sigal
通讯作者：
Balshine, Sigal
影响因子：
1.9
作者：
Ho, Katharine;Wolff, Collin A.;Suarez, Susan S.
通讯作者：
Suarez, Susan S.
影响因子：
10.7
作者：
Montgomery, Stephen H.;Capellini, Isabella;Mundy, Nicholas I.
通讯作者：
Mundy, Nicholas I.
影响因子：
3.6
作者：
Loux, Shavahn C.;Crawford, Kristin R.;Hinrichs, Katrin
通讯作者：
Hinrichs, Katrin