Unravelling cadmium toxicity and nitric oxide induced tolerance in Cucumis sativus: Insight into regulatory mechanisms using proteomics
Unravelling cadmium toxicity and nitric oxide induced tolerance in Cucumis sativus: Insight into regulatory mechanisms using proteomics
复制标题
解开黄瓜中镉毒性和一氧化氮诱导的耐受性：利用蛋白质组学洞察调控机制
DOI：
10.1016/j.jhazmat.2017.04.058
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发表时间：
2017-08-15
影响因子：
13.6
通讯作者：
Shi, Qinghua
中科院分区：
文献类型：
--
作者：
Gong, Biao;Nie, Wenjing;Shi, Qinghua
一氧化氮（NO）是一种信号分子，可以介导植物抗镉（Cd）毒性的一系列生理过程。然而，一氧化氮对黄瓜抗Cd胁迫能力的全局和系统的减缓机制却知之甚少。本研究利用iTRAQ （Isobaric Tag for Relative and Absolute Quantification）技术鉴定了1691个蛋白质，这些蛋白质可用于确定NO在镉胁迫下黄瓜叶片蛋白质组分子变化中的调节作用。几种关键蛋白的失调表明cd诱导黄瓜叶片的生理退化主要涉及代谢过程、细胞过程、对刺激的反应等。代谢途径分析表明，NO处理显著逆转了Cd破坏途径，包括Cd运输和定位、光合作用、叶绿素代谢、氧化还原稳态、谷胱甘肽介导的Cd解毒和Ca2+信号转导。综上所述，该iTRAQ分析为黄瓜NO抗Cd毒性的生理和分子机制提供了更全面的见解。(C) 2017 Elsevier B.V.版权所有
Nitric oxide (NO) is a signal molecule that can mediate a wide range of physiological processes against cadmium (Cd) toxicity in plants. However, little information can be used to reveal the global and systematic mitigative mechanism of NO in improving Cd stress tolerance of cucumber plants. In the present study, we used Isobaric Tag for Relative and Absolute Quantification (iTRAQ) analysis to identify 1691 proteins, which can be used to determine the role of NO in regulating the molecular changes of proteome in cucumber leaves exposed to Cd stress. Several dysregulated key proteins indicated that Cd-induced physiological deterioration of cucumber leaves were mainly involved in metabolic process, cellular process, response to stimulus and so on. Metabolic pathway analysis indicated that several Cd-disruptive pathways were markedly reversed by NO treatments, including Cd transport and localization, photo-synthesis, chlorophyll metabolism, redox homeostasis, glutathione-mediated Cd detoxification and Ca2+ signaling transduction. Taken together, this iTRAQ analysis provides more comprehensive insights into the physiological and molecular mechanisms of NO against Cd toxicity in cucumber plants. (C) 2017 Elsevier B.V. All rights reserved.