Translational Components Contribute to Acclimation Responses to High Light, Heat, and Cold in Arabidopsis
Translational Components Contribute to Acclimation Responses to High Light, Heat, and Cold in Arabidopsis
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DOI：
10.1016/j.isci.2020.101331
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发表时间：
2020-07-24
期刊：
影响因子：
5.8
通讯作者：
Leister, Dario
中科院分区：
文献类型：
--
作者：
Garcia-Molina, Antoni;Kleine, Tatiana;Leister, Dario
植物在适应非生物变化的过程中，其代谢会发生广泛的重组。大多数以前的研究都集中在从标准到单一压力条件的过渡。在这里，我们系统地分析驯化过程中的光，热，冷应力的水平，微妙地改变生理参数，并评估其可逆性在去适应。在11个不同的时间点监测代谢组和转录组的变化。与转录组变化不同，代谢物水平的大多数变化不容易恢复到基线值，除了在冷驯化的情况下。类似的监管网络在（去）适应高光和冷，而热和高光响应表现出类似的动态，如通过系统和条件网络分析确定的。在这里测试的所有驯化模型中，条件转录组网络中的超级枢纽富含参与翻译的组件，特别是核糖体。因此，我们认为，核糖体作为一个共同的中央枢纽控制三个不同的（去）适应反应。
Plant metabolism is broadly reprogrammed during acclimation to abiotic changes. Most previous studies have focused on transitions from standard to single stressful conditions. Here, we systematically analyze acclimation processes to levels of light, heat, and cold stress that subtly alter physiological parameters and assess their reversibility during de-acclimation. Metabolome and transcriptome changes were monitored at 11 different time points. Unlike transcriptome changes, most alterations in metabolite levels did not readily return to baseline values, except in the case of cold acclimation. Similar regulatory networks operate during (de-)acclimation to high light and cold, whereas heat and high-light responses exhibit similar dynamics, as determined by surprisal and conditional network analyses. In all acclimation models tested here, super-hubs in conditional transcriptome networks are enriched for components involved in translation, particularly ribosomes. Hence, we suggest that the ribosome serves as a common central hub for the control of three different (de-)acclimation responses.