Alternative procedure for charged derivatization to enhance detection responses of steroids in electrospray ionization-MS
Alternative procedure for charged derivatization to enhance detection responses of steroids in electrospray ionization-MS
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DOI：
10.1248/cpb.55.662
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发表时间：
2007-04-01
影响因子：
1.7
通讯作者：
Shimada, Kazutake
中科院分区：
文献类型：
--
作者：
Higashi, Tatsuya;Nishio, Tadashi;Shimada, Kazutake
一种衍生化程序已被检查，以提高电喷雾电离(ESI)-质谱检测类固醇，带电衍生不适合。2-肼吡啶衍生化或异烟碱叠氮化反应分别对二氧类固醇和二羟基类固醇的检测非常有效，所得衍生物的检出限低至约2 fmol。该衍生物还在质谱联用中提供了强烈的特征产物离子，有望用于选定的反应监测模式。
A derivatization procedure has been examined to enhance the electrospray ionization (ESI)-MS detectabilities of steroids that charged derivatization is not suitable for. The derivatization procedure with 2-hydrazinopyridine or isonicotinoyl azide was very effective for the sensitive detection of di-oxosteroids or di-hydroxysteroids, respectively, and the detection limits of the resulting derivatives were as low as about 2 fmol. The derivatives also provided intense characteristic product ions in the MS-MS, which are expected to be usable for the selected reaction monitoring mode.