A rapid procedure for purifying IgM monoclonal antibodies from murine ascites using a DEAE-disk.
A rapid procedure for purifying IgM monoclonal antibodies from murine ascites using a DEAE-disk.
复制标题
使用 DEAE 盘从小鼠腹水中纯化 IgM 单克隆抗体的快速程序。
作者：
Burd,RS;Raymond,CS;Ratz,CA;Dunn,DL
A method for purifying IgM monoclonal antibodies (mAbs) from murine ascites using a DEAE-disk is described. After ammonium sulfate precipitation, ascites proteins are redissolved and loaded onto a DEAE-disk. MAb then is eluted from the disk using a stepwise NaCl gradient. IgM mAb produced by this procedure was >95% pure as assessed by reducing SDS-PAGE analysis and was free of significant IgG contamination as determined by double radial immunodiffusion analysis. Yield of IgM mAb was ̃2% of total ascites protein and ̃10% of the amount of IgM contained in crude ascites. MAb retained immunoreactivity as assessed by ELISA, and the affinity index was evaluated by thiocyanate elution and remained unchanged. This two step technique for purifying IgM mAb from murine ascites is rapid, simple, and yields mAb of sufficient purity and immunoreactivity for the majority of mAb applications.
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影响因子：
2.2
作者：
MACDONALD, RA;HOSKING, CS;JONES, CL
通讯作者：
JONES, CL
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影响因子：
4.1
作者：
H. H. Hwang;M. Healey;A. V. Johnston
通讯作者：
A. V. Johnston
影响因子：
3.9
作者：
SHIBUYA, N;BERRY, JE;GOLDSTEIN, IJ
通讯作者：
GOLDSTEIN, IJ
用针对脂多糖的鼠单克隆抗体治疗实验性革兰氏阴性细菌败血症。
影响因子：
3.8
作者：
Priest,BP;Brinson,DN;Schroeder,DA;Dunn,DL
通讯作者：
Dunn,DL
使用蓝色 DEAE 和亲硫吸附剂从腹水中 FPLC 纯化小鼠单克隆抗体。
DOI：
--
发表时间：
1991
期刊：
JIM - Journal of Immunological Methods
影响因子：
--
作者：
E. Juronen;J. Parik;P. Toomik
通讯作者：
P. Toomik