TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?
TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?
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DOI：
10.1016/j.mimet.2015.05.005
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发表时间：
2015-07-01
影响因子：
2.2
通讯作者：
Epstein, Slava S.
中科院分区：
文献类型：
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作者：
Sizova, Maria-V.;Doerfert, Sebastian N.;Epstein, Slava S.
TM7 显得重要且无所不在，因为它在不同的环境中被分子技术反复检测到。在这里，我们报告大多数被认为是 TM7 特异性的引物和 FISH 探针确实与口腔和阴道腔的多种物种杂交。这需要重新检查 TM7 的分布和丰度。 (C) 2015 年由 Elsevier B.V. 出版
TM7 appears important and omnipresent because it is repeatedly detected by molecular techniques in diverse environments. Here we report that most of primers and FISH probes thought to be TM7-specific do hybridize with multiple species from oral and vaginal cavity. This calls for re-examination of TM7 distribution and abundance. (C) 2015 Published by Elsevier B.V.