PROTEIN-SYNTHESIS IN LONG-TERM STATIONARY-PHASE CULTURES OF SACCHAROMYCES-CEREVISIAE
PROTEIN-SYNTHESIS IN LONG-TERM STATIONARY-PHASE CULTURES OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/jb.176.18.5802-5813.1994
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发表时间：
1994-09-01
影响因子：
3.2
通讯作者：
WERNERWASHBURNE, M
中科院分区：
文献类型：
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作者：
FUGE, EK;BRAUN, EL;WERNERWASHBURNE, M
我们感兴趣的是表征进入和维持固定相的过程。为了鉴定在生长到稳定期期间诱导的蛋白质，我们使用二维聚丙烯酰胺凝胶电泳（2D-PAGE）检查了长期稳定期培养物中的蛋白质合成。虽然蛋白质合成的总速率下降时，停止后的postdiauxic阶段的增长，蛋白质合成的模式在整个实验期间（28天）保持相似，除了在diauxic转变。在二次生长的转变，大多数蛋白质可检测的2D-PAGE经历一个短暂的减少，其相对合成率结束时，细胞恢复生长在postdiauxic阶段。我们由此得出结论，蛋白质合成的瞬时抑制在二次分裂的转变是不直接相关的静止相逮捕。已经通过2D-PAGE鉴定了许多在指数期后合成的蛋白质。这些蛋白质可以分为三个时间类，这取决于它们的合成何时变得可检测。一个指数后的蛋白质，指定p35，诱导晚于所有其他蛋白质，其相对合成率增加整个稳定期。与大多数的postexponential蛋白质，p35不受热休克或葡萄糖阻遏。我们还观察到，稳态mRNA积累和蛋白质合成的另一个postexponential蛋白（Ssa 3 p）或两个密切相关的组成蛋白（Ssa 1 p和Ssa 2 p）之间的直接相关性不存在。我们得出结论，从这个结果，在稳定期的蛋白质合成的调节机制以外的控制稳态mRNA的积累。
We are interested in characterizing the process of entry into and the maintenance of the stationary phase. To identify proteins that are induced during growth to stationary phase, we examined protein synthesis in long-term stationary-phase cultures using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Although the total rate of protein synthesis declined when growth ceased after the postdiauxic phase, the pattern of proteins synthesized remained similar throughout the experimental period (28 days), except at the diauxic shift. At the diauxic shift most proteins detectable by 2D-PAGE undergo a transient reduction in their relative rate of synthesis that ends when cells resume growth during the postdiauxic phase. We conclude from this that the transient repression of protein synthesis at the diauxic shift is not directly associated with stationary-phase arrest. A number of proteins that are synthesized after exponential phase have been identified by 2D-PAGE. These proteins could be divided into three temporal classes depending upon when their synthesis became detectable. One postexponential protein, designated p35, was induced later than all other proteins, and its relative rate of synthesis increased throughout stationary phase. Unlike most postexponential proteins, p35 was not regulated by heat shock or glucose repression. We also observed that a direct correlation between steady-state mRNA accumulation and protein synthesis for another postexponential protein (Ssa3p) or two closely related constitutive proteins (Ssa1p and Ssa2p) did not exist. We conclude from this result that synthesis of proteins in stationary phase is regulated by mechanisms other than the control of steady-state mRNA accumulation.