PHOSPHATE-DEPENDENT MONOCLONAL-ANTIBODIES TO NEUROFILAMENTS AND ALZHEIMER NEUROFIBRILLARY TANGLES RECOGNIZE A SYNTHETIC PHOSPHOPEPTIDE
PHOSPHATE-DEPENDENT MONOCLONAL-ANTIBODIES TO NEUROFILAMENTS AND ALZHEIMER NEUROFIBRILLARY TANGLES RECOGNIZE A SYNTHETIC PHOSPHOPEPTIDE
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DOI：
10.1111/j.1471-4159.1990.tb01203.x
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发表时间：
1990-05-01
影响因子：
4.7
通讯作者：
ANDERTON, BH
中科院分区：
文献类型：
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作者：
COLEMAN, MP;ANDERTON, BH
五个磷酸依赖性单克隆抗体的神经丝重链多肽结合强烈的磷酸化的合成肽，其中包含一个单一的Lys-Ser-Pro序列，在人类神经丝。其中三种抗体标记了阿尔茨海默病神经元缠结，两种没有，这表明在缠结中，与肽相似的表位可用于一些但不是所有抗体。此外，发现一些抗体比其他抗体更受抗原的酶促去磷酸化的影响，但是因为所有抗体结合相同的合成磷酸肽，所以它们不结合相互排斥的磷酸化位点。相反，磷酸盐依赖性抗体可能更直接地结合磷酸基团，正如它们被无机磷酸盐和游离磷酸丝氨酸取代所表明的那样。
Five phosphate-dependent monoclonal antibodies to the neurofilament heavy polypeptide bound strongly to a phosphorylated synthetic peptide, which contains a single Lys-Ser-Pro sequence tht occurs in human neurofilaments. Three of the antibodies label Alzheimer''s disease neurofibrillary tangles and two do not, suggesting that in tangles an epitope similar to the peptide is available to some but not all of the antibodies. In addition, some antibodies were found to be more affected than others by enzymatic dephosphorylation of the antigen, but because all the antibodies bound the same synthetic phosphopeptide they do not bind to mutually exclusive phosphorylation sites. Instead the more phosphate-dependent antibodies might bind the phosphate-dependent antibodies might bind the phosphate group more directly, as suggested by their inhibiton by inorganic phosphate and free phosphoserine.