SipA, SopA, SopB, SopD, and SopE2 contribute to Salmonella enterica serotype Typhimurium invasion of epithelial cells
SipA, SopA, SopB, SopD, and SopE2 contribute to Salmonella enterica serotype Typhimurium invasion of epithelial cells
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DOI：
10.1128/iai.73.1.146-154.2005
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发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
Bäumler, AJ
中科院分区：
文献类型：
--
作者：
Raffatellu, M;Wilson, RP;Bäumler, AJ
由沙门氏菌致病岛1（SPI 1）编码的第三型分泌系统（T3 SS-1）介导肠道沙门氏菌血清型鼠伤寒对上皮细胞的侵袭。缺乏单个基因的突变体的表征揭示了T3 SS-1分泌的蛋白（效应子）SopE 2和SopB是入侵所需的，而SipA蛋白加速进入细胞。在这里，我们重新讨论了T3 SS-1效应子通过补充缺乏引起小牛腹泻所需的所有效应子基因（sipA sopABDE 2突变体）的菌株来促进上皮细胞入侵的问题。无论是克隆sipA，克隆sopB，或克隆sopE 2基因的引入增加了sipA sopABDE 2突变体的侵袭力。非极化HT-29细胞。然而，当用非极化结肠癌细胞系T84和HT-29进行侵袭测定时，SOPA或SOPD对侵袭的贡献并不明显。相反，sopA、sopB、sopD或sopE 2基因的引入增加了sipA sopABDE 2突变体对极化T84细胞的侵袭力。此外，与仅携带sipA或sopB的质粒的引入相比，引入携带sipA和sopB的质粒显著增加了sipA sopABDE 2突变体对极化T84细胞的侵袭力。我们得出结论，SipA，SopA，SopB，SopD和SopE 2有助于S。肠血清型鼠伤寒杆菌体外侵袭上皮细胞。
The centisome 63 type III secretion system (T3SS-1) encoded by Salmonella pathogenicity island 1 (SPI1) mediates invasion of epithelial cells by Salmonella enterica serotype Typhimurium. Characterization of mutants lacking individual genes has revealed that T3SS-1 secreted proteins (effectors) SopE2 and SopB are required for invasion while the SipA protein accelerates entry into cells. Here we have revisited the question of which T3SS-1 effectors contribute to the invasion of epithelial cells by complementing a strain lacking all of the effector genes that are required to cause diarrhea in a calf (a sipA sopABDE2 mutant). Introduction of either the cloned sipA, the cloned sopB, or the cloned sopE2 gene increased the invasiveness of the sipA sopABDE2 mutant. for nonpolarized HT-29 cells. However, a contribution of sopA or sopD to invasion was not apparent when invasion assays were performed with the nonpolarized colon carcinoma cell lines T84 and HT-29. In contrast, introduction of either the sopA, the sopB, the sopD, or the sopE2 gene increased the invasiveness of the sipA sopABDE2 mutant for polarized T84 cells. Furthermore, introduction of a plasmid carrying sipA and sopB increased the invasiveness of the sipA sopABDE2 mutant for polarized T84 cells significantly compared to the introduction of plasmids carrying only sipA or sopB. We conclude that SipA, SopA, SopB, SopD, and SopE2 contribute to S. enterica serotype Typhimurium invasion of epithelial cells in vitro.