Applicability of the use of charcoal for the evaluation of intestinal motility in a murine model of Trypanosoma cruzi infection
Applicability of the use of charcoal for the evaluation of intestinal motility in a murine model of Trypanosoma cruzi infection
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DOI：
10.1007/s00436-007-0829-8
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发表时间：
2008-03-01
影响因子：
2
通讯作者：
de Souza, Andrea Pereira
中科院分区：
文献类型：
--
作者：
de Oliveira, Gabriel Melo;Medeiros, Monica de Melo;de Souza, Andrea Pereira
查加斯病是由原生动物克氏锥虫引起的，在拉丁美洲仍然是一个严重的公共卫生问题。在消化问题方面，4.5%的患者在慢性期出现巨型综合征(巨结肠)。在这篇文章中，我们通过木炭摄取成年小鼠来评估克氏毛滴虫感染急性期的肠道运动。感染后，瑞士小鼠被灌胃给予水中木炭的水悬浮液。肠道动力的下降是由粪便中木炭出现的时间增加所确定的。在整个实验过程中，未感染组的平均碳排出时间为109.0±14.6min。另一方面，感染的小鼠在感染期间木炭排便时间显著增加。感染15d后，感染组小鼠的炭末排便时间为310.2±67.4min，明显长于未感染组的97.8min/-31.8min，表明克氏毛滴虫感染干扰了小鼠的肠道运动。我们的结果表明，在小鼠感染毛滴虫的模型中，使用木炭是一种道德和有效的评估肠道运动的方法。
Chagas disease, caused by the protozoan Trypanosoma cruzi, remains a serious public health problem in Latin America. In relation to digestive problems, 4.5% of patients show mega syndromes (megacolon) in the chronic phase. In this article, we evaluated intestinal motility at the acute phase of T. cruzi infection through charcoal ingestion in adult mice. After infection, Swiss mice were administered an aqueous suspension of charcoal in water by gavage. Decrease in intestinal motility was determined by increased time of appearance of charcoal in the feces. The uninfected group showed a mean time of charcoal elimination of 109.0 +/- 14.6 min throughout the assay. On the other hand, infected mice presented a significant increase in charcoal defecation time during infection. At 15 days postinfection, infected mice showed a significant increase in charcoal defecation time, 310.2 +/- 67.4 min when compared to the uninfected group, which presented 97.8 +/- 31.8 min, indicating that the T. cruzi infection interferes with intestinal motility. Our results demonstrate that the use of charcoal is an ethical and efficient procedure to evaluate the intestinal motility in the murine model of T. cruzi infection.