Subinhibitory Concentrations of Allicin Decrease Uropathogenic Escherichia coli (UPEC) Biofilm Formation, Adhesion Ability, and Swimming Motility.
Subinhibitory Concentrations of Allicin Decrease Uropathogenic Escherichia coli (UPEC) Biofilm Formation, Adhesion Ability, and Swimming Motility.
复制标题
亚抑制浓度的大蒜素可降低尿路致病性大肠杆菌 (UPEC) 生物膜的形成、粘附能力和游动能力
DOI：
10.3390/ijms17070979
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发表时间：
2016-06-29
影响因子：
5.6
通讯作者：
Huang N
中科院分区：
文献类型：
--
作者：
Yang X;Sha K;Xu G;Tian H;Wang X;Chen S;Wang Y;Li J;Chen J;Huang N
尿路致病性大肠杆菌（UPEC）生物膜的形成使生物体能够避开宿主免疫系统，抵抗抗生素，并为持续感染提供了一个水库。一旦生物膜建立，根除感染变得困难。因此，迫切需要针对UPEC生物膜的策略。在这项研究中，我们研究了大蒜素，从大蒜精油中分离，对UPEC CFT 073和J 96生物膜的形成和分散的影响，沿着其对UPEC粘附能力和游泳运动的影响。亚抑菌浓度（亚MIC）的大蒜素减少UPEC生物膜的形成，并影响其结构。大蒜素也具有分散生物膜的能力。此外，大蒜素降低细菌的粘附能力和游泳运动，这是重要的生物膜的形成。实时荧光定量聚合酶链反应（RT-qPCR）结果显示，大蒜素可降低UPEC 1型菌毛粘附素基因fimH的表达。对接研究表明，大蒜素位于FimH中庚基α-d-甘露吡喃糖苷的结合口袋内，与Phe 1和Asn 135形成氢键。此外，大蒜素降低了UPEC CFT 073的双组分调节系统（TCS）同源反应调节基因uvrY的表达，并增加了与UPEC生物膜相关的RNA结合全局调节蛋白基因csrA的表达。结果表明，大蒜素的亚MIC能够影响UPEC生物膜的形成和分散，并降低UPEC的粘附能力和游泳运动。
Uropathogenic Escherichia coli (UPEC) biofilm formation enables the organism to avoid the host immune system, resist antibiotics, and provide a reservoir for persistent infection. Once the biofilm is established, eradication of the infection becomes difficult. Therefore, strategies against UPEC biofilm are urgently required. In this study, we investigated the effect of allicin, isolated from garlic essential oil, on UPEC CFT073 and J96 biofilm formation and dispersal, along with its effect on UPEC adhesion ability and swimming motility. Sub-inhibitory concentrations (sub-MICs) of allicin decreased UPEC biofilm formation and affected its architecture. Allicin was also capable of dispersing biofilm. Furthermore, allicin decreased the bacterial adhesion ability and swimming motility, which are important for biofilm formation. Real-time quantitative polymerase chain reaction (RT-qPCR) revealed that allicin decreased the expression of UPEC type 1 fimbriae adhesin gene fimH. Docking studies suggested that allicin was located within the binding pocket of heptyl α-d-mannopyrannoside in FimH and formed hydrogen bonds with Phe1 and Asn135. In addition, allicin decreased the expression of the two-component regulatory systems (TCSs) cognate response regulator gene uvrY and increased the expression of the RNA binding global regulatory protein gene csrA of UPEC CFT073, which is associated with UPEC biofilm. The findings suggest that sub-MICs of allicin are capable of affecting UPEC biofilm formation and dispersal, and decreasing UPEC adhesion ability and swimming motility.