Functional evaluation of the grafted wall with porcine-derived small intestinal submucosa (SIS) to a stomach defect in rats
Functional evaluation of the grafted wall with porcine-derived small intestinal submucosa (SIS) to a stomach defect in rats
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DOI：
10.1016/j.surg.2007.04.019
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发表时间：
2007-09-01
期刊：
影响因子：
3.8
通讯作者：
Pappas, Theodore N.
中科院分区：
文献类型：
--
作者：
Ueno, Tomio;de la Fuente, Sebastian G.;Pappas, Theodore N.
背景小肠黏膜下层（SIS）是一种新型的生物支架材料，可用于修复中空器官缺损。然而，目前还不清楚神经生理反应是否返回到肠中的SIS移植区域。我们评估了移植了猪源性SIS的胃缺损的功能恢复。12只大鼠在胃中产生全层缺损。将SIS固定在胃壁上。6个月后，从移植区域内收获肌条进行组织学和功能研究。从同一胃的后部采集额外的全层肌条作为对照。用氨甲酰胆碱（CCH）或硝普钠（SNP）获得剂量反应曲线。用电场刺激（EFS）激活内源性神经。对照组和SIS条对CCH的反应和EFS的振幅均呈浓度依赖性和频率依赖性的离子收缩。每次刺激后的幅度在SIS条中显著低于对照组（P <0.01）。而E-PS收缩率与CCH收缩率的比值无显著性差异。此外，SNP以浓度依赖性方式在两条中产生松弛。组织学结果显示，固有肌层中平滑肌细胞数量不足，而粘膜下层中观察到补偿性生长和神经再生。这项研究表明，SIS提供了一个模板的神经迁移到移植物在啮齿动物的胃。神经支配表现出类似的分布，在控制中观察到的。这些发现的临床意义需要进一步的研究。
Background. Small intestinal submucosa (SIS) represents a novel bio-scaffolding material that may be used to repair hollow-organ defects. However, it is unclear whether neurophysiologic responses return to SIS-grafted areas in the gut. We evaluated the functional recovery of a stomach defect grafted with the porcine-derived SIS.Methods. Twelve rats had a full-thickness defect created in the stomach. SIS was secured to the gastric wall. After 6 months, muscle strips were harvested from within the grafted area to perform both a histologic and a functional study. Additional full-thickness muscle strips were harvested from the posterior in the same stomach as controls. A dose response curve was obtained with carbachol (CCH) or sodium nitroprusside (SNP). Activation of intrinsic nerves was achieved by electrical field stimulation (EFS).Results. The response to CCH and amplitude in EFS showed Ionic contraction in both controls and SIS strips in a concentration-dependent and frequency-dependent manner. The magnitude after each stimulation was significantly lower in SIS strips compared with controls (P < .01). However, the contraction ratio of E-PS to ED50 of CCH was not significantly different between the groups. Additionally, SNP produced relaxation in both strips in a concentration-dependent manner. Histologic findings revealed that an insufficient amount of smooth-muscle cells existed in the muscularis propria, whereas compensated growth was observed in the submucosa with nerve regeneration.Conclusions. This study demonstrates that SIS provides a template for nerve migration to the graft in the rodent stomach. Innervations showed a similar distribution to that observed in the controls. The clinical implications of such findings warrant additional investigation.