Swine influenza virus strains recognize sialylsugar chains containing the molecular species of sialic acid predominantly present in the swine tracheal epithelium
Swine influenza virus strains recognize sialylsugar chains containing the molecular species of sialic acid predominantly present in the swine tracheal epithelium
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DOI：
10.1016/s0014-5793(97)00127-0
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发表时间：
1997-03-10
期刊：
影响因子：
3.5
通讯作者：
Suzuki, Y
中科院分区：
文献类型：
--
作者：
Suzuki, T;Horiike, G;Suzuki, Y
我们用荧光高效液相色谱法测定了猪呼吸道上皮细胞中N-羟乙酰神经氨酸（Neu 5Gc）与N-乙酰神经氨酸（Neu 5Ac）的比例，并检测了猪流感病毒株与含有不同分子种类唾液酸的神经节苷脂的结合特异性（Neu 5Ac和Neu 5Gc），以及含有Neu 5Ge作为其主要唾液酸的牛红细胞唾液酸糖蛋白2（GP-2）（96%的总唾液酸），Neu 5Gc（在人气管上皮中未检测到）和Neu 5Ac（在猪气管上皮中）的存在在1：除A/swine/爱荷华州/15/30（H1N1）外，所测试的猪流感病毒H1和H3分离株与人流感病毒株相比显示出对含有Neu 5Gc的唾液酸糖链的显著结合能力，这些结果表明，猪流感病毒识别主要存在于猪气管上皮中的唾液酸分子种类的唾液酸糖链。(C)1997年欧洲生物化学学会联合会。
We determined the ratio of N-glycolylneuraminic acid (Neu5Gc) to N-acetylneuraminic acid (Neu5Ac) in swine respiratory epithelia by fluorometric high-performance liquid chromatography, and examined the binding specificity of swine influenza virus strains for gangliosides containing different molecular species of sialic acid (Neu5Ac and Neu5Gc), and for bovine erythrocyte sialoglycoprotein 2 (GP-2) containing Neu5Ge as its predominate sialic acid (96% of total sialic acids), The presence of Neu5Gc, which had not been detected in human tracheal epithelia, and Neu5Ac in swine tracheal epithelia was observed in a 1:1 ratio, The swine influenza virus H1 and H3 isolates tested, except for A/swine/Iowa/15/30 (H1N1), displayed a marked binding ability for sialylsugar chains containing Neu5Gc compared with that of the human influenza virus strains, These results suggest that swine influenza viruses recognize sialylsugar chains containing the molecular species of sialic acid present predominantly in the swine tracheal epithelium. (C) 1997 Federation of European Biochemical Societies.