Cimicifuga and Melbrosia lack oestrogenic effects in mice and rats
Cimicifuga and Melbrosia lack oestrogenic effects in mice and rats
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DOI：
10.1016/0378-5122(96)01052-3
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发表时间：
1996-10-01
期刊：
影响因子：
4.9
通讯作者：
Kristoffersen, K
中科院分区：
文献类型：
--
作者：
EinerJensen, N;Zhao, J;Kristoffersen, K
目的：天然药物升麻和大头草的广泛销售。升麻是总状升麻（L.）的提取物，而Melbrosia是Gelee Royale、perga-pollen和花粉的混合物。许多咨询私人妇科医生的丹麦女性通过自我用药来缓解潮热和其他经期或更年期不适症状。妇科医生倾向于用雌激素来治疗这些症状，因此本实验旨在研究升麻和大花草是否具有经典生物学方法所定义的雌激素作用：未成熟小鼠的子宫生长和卵巢切除大鼠的阴道角化。方法：将媒介物、6、60或600 mg/kg升麻或30、300或3000 mg/kg Melbrosia口服给予10只未成熟小鼠组3天，并在第四天测量子宫重量。类似地，将媒介物、6、60、600 mg/kg升麻或3、30、300 mg/kg Melbrosia皮下注射到12只切除卵巢的大鼠组中3天，并检查阴道涂片是否有角化细胞的迹象。所有实验均重复一次。结果：在任何实验中均未发现与制剂有关的雌激素作用迹象。结论： II 可以得出这样的结论：与升麻和大花香自我药疗有关的对月经或更年期不适的最终有益作用不能解释为生物实验中测量到的传统雌激素作用。
Objectives: The natural medicines, Cimicifuga and Melbrosia, are widely sold. Cimicifuga is an extract of Cimicifuga racemosa (L.), and Melbrosia is a mixture of Gelee Royale, perga-pollen and pollen. Cimicifuga and Melbrosia are used through self-medication to relieve symptoms of hot flushes and other menstrual or menopausal discomfort in many of the Danish women consulting private gynaecologists. A gynaecologist tends to treat these symptoms with oestrogen, so the present experiments were therefore made to investigate whether Cimicifuga and Melbrosia have oestrogenic effects as defined by the classical biological methods: uterine growth in immature mice and vaginal cornification in ovariectomized rats. Methods: Vehicle, 6, 60 or 600 mg/kg Cimicifuga or 30, 300 or 3000 mg/kg Melbrosia was administered orally for 3 days to groups of 10 immature mice and the uterus weight measured on the fourth day. Similarly, vehicle, 6, 60, 600 mg/kg Cimicifuga or 3, 30, 300 mg/kg Melbrosia was injected subcutaneously in groups of 12 ovariectomized rats for 3 days and vaginal smears investigated for signs of cornified cells. All experiments were repeated once. Results: No signs of an oestrogenic effect connected with the preparations were found in any of the experiments. Conclusions: II can be concluded that the eventual beneficial effects on menstrual or menopausal discomfort connected with Cimicifuga and Melbrosia self-medication cannot be explained as a traditional oestrogenic effect as measured in biological experiments.