Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress
Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress
复制标题
冬冬青半乳糖醇合酶在番茄中的表达提高了对冷胁迫的耐受性
DOI：
10.1093/jxb/erz450
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发表时间：
2020-01-01
影响因子：
6.9
通讯作者：
Li, TianLai
中科院分区：
文献类型：
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作者：
Liu, YuDong;Zhang, Li;Li, TianLai
可溶性碳水化合物不仅直接影响植物的生长发育，而且在植物抗冷胁迫过程中作为信号分子。棉子糖家族寡糖（RFOs）就是一个例子，在非生物胁迫耐受中起着重要作用。本研究旨在确定半乳糖醇作为RFO生物合成的关键限制因子，是否作为信号分子在触发耐寒性中起作用。低温条件下，荒漠植物Ammopiptanthus nanus中半乳糖醇合成酶（AnGolS1）的表达明显高于番茄半乳糖醇合成酶（SlGolS2）。此外，SlGolS2仅在低水平表达。AnGoIS1在番茄中的表达增强了番茄的耐寒性，并导致了种子和幼苗中糖成分的变化。AnGolS1转基因番茄株系表现出增强的乙烯（ET）信号传导能力。半乳糖醇的应用消除了种子萌发过程中1-甲基环丙烯对ET信号通路的抑制。此外，ERF转录因子的表达增加。因此，半乳糖醇可能作为影响ET通路的信号分子。
Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress. Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance. This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance. Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 degrees C to 47 degrees C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2). Moreover, SlGolS2 is expressed only at low levels. Expression of AnGoIS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings. AnGolS1 transgenic tomato lines exhibited an enhanced capacity for ethylene (ET) signaling. The application of galactinol abolished the repression of the ET signaling pathway by 1-methylcyclopropene during seed germination. In addition, the expression of ERF transcription factors was increased. Galactinol may therefore act as a signal molecule affecting the ET pathway.