A NEW HEAT-LABILE CYTOLETHAL DISTENDING TOXIN (CLDT) PRODUCED BY ESCHERICHIA-COLI ISOLATES FROM CLINICAL MATERIAL
A NEW HEAT-LABILE CYTOLETHAL DISTENDING TOXIN (CLDT) PRODUCED BY ESCHERICHIA-COLI ISOLATES FROM CLINICAL MATERIAL
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DOI：
10.1016/0882-4010(88)90052-6
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发表时间：
1988-02-01
影响因子：
3.8
通讯作者：
LIOR, H
中科院分区：
文献类型：
--
作者：
JOHNSON, WM;LIOR, H
在43种与腹泻相关的大肠杆菌菌株的培养滤液中发现了一种新的热不稳定的大肠杆菌毒素，对Vero、HeLa、HEp-2和CHO细胞具有毒性，对Y-1细胞呈阴性。这种新毒素被称为细胞致死扩张毒素（CLDT），以反映所有敏感组织细胞的进行性细胞扩张和细胞毒性。CLDT不同于经典的热不稳定型（LT）和热稳定型肠毒素、vero毒素和溶血素，它是由以下大肠杆菌血清群（02、07、08、018、022、039、044、055、083、0.86、091、0113、0119、0128和0167）中的一些菌株产生的。在暴露于大肠杆菌CLDT 24小时的CHO细胞中观察到中度循环AMP积累（75倍）。与大肠杆菌LT相比，CHO细胞暴露于大肠杆菌CLDT后的72小时内，循环AMP水平在24小时达到最佳水平，并在随后的72小时内观察到降低。除了耐热性在70度。C 15分钟，大肠杆菌CLDT显示分子量超过30,000，不可透析和胰蛋白酶敏感。大肠杆菌CLDT在成年家兔结扎回肠环和乳鼠试验中呈阴性，仅在家兔皮肤中引起红斑反应。迄今为止鉴定出的所有cldt阳性大肠杆菌菌株都是非溶血性和非侵入性的。在少数大肠杆菌中发现了与CLDT结合的维罗毒素和热不稳定肠毒素。
A new heat-labile Escherichia coli toxin cytolethal to Vero, HeLa, HEp-2 and CHO cells and negative in Y-1 cells has been demonstrated in culture filtrates of 43 E. coli strains associated with diarrheal disease. This new toxin was termed a cytolethal distending toxin (CLDT) to reflect the progressive cell distension and cytotoxicity evidenced in all sensitive tissue cells. CLDT was distinct from the classic heat-labile (LT) and heat-stable enterotoxins, Verotoxins and hemolysins and was produced by some strains of the following E. coli serogroups (02, 07, 08, 018, 022, 039, 044, 055, 083, 0.86, 091, 0113, 0119, 0128 and 0167). Moderate cyclic AMP accumulation (75-fold) was observed in CHO cells exposed to E. coli CLDT for 24 h. In contrast to E. coli LT, cyclic AMP levels were optimal at 24 h and were observed to decrease over the following 72 h period in CHO cells exposed to E. coli CLDT. In addition to heat-lability at 70.degree. C for 15 min, E. coli CLDT demonstrated a molecular weight over 30,000, was nondialyzable and trypsin-sensitive. E. coli CLDT was negative in adult rabbit ligated ileal loop and suckling mouse assays and provoked only an erythematous response in rabbit skin. All CLDT-positive E. coli strains identified to date were non-hemolytic and non-invasive. Verotoxin and heat-labile enterotoxin were found in combination with CLDT in a few E. coli strains.