Production of feruloyl esterases and xylanases by Talaromyces stipitatus and Humicola grisea var. thermoidea on industrial food processing by-products.
Production of feruloyl esterases and xylanases by Talaromyces stipitatus and Humicola grisea var. thermoidea on industrial food processing by-products.
复制标题
DOI：
10.1016/j.biortech.2007.09.022
复制
发表时间：
2008-07
影响因子：
11.4
通讯作者：
G. Mandalari;G. Bisignano;R. L. Lo Curto;K. Waldron;C. Faulds
中科院分区：
文献类型：
--
作者：
G. Mandalari;G. Bisignano;R. L. Lo Curto;K. Waldron;C. Faulds
对稻瘟病菌培养上清液中的阿魏酸酯酶和木聚糖酶活性进行了检测。以啤酒糟(BSG)和麦皮(WB)这两种农工业副产品为原料生长的Theridae和Talarmyces stipiatus。在150rpm的培养条件下，稻瘟病菌的木聚糖酶活力最高，BSG和WB的木聚糖酶活力分别为16.9U/ml和9.1U/ml。BSG和WB的最大FAE活性分别为0.47U/ml和0.33U/ml。对微生物生长后残留的细胞壁物质的分析表明，阿拉伯木聚糖和纤维素是BSG和WB中存在的两种主要多糖。利用农用工业副产品生产低成本的细胞壁解构酶可导致生产用于食品加工废物有价化的低成本酶。
Feruloyl esterase (FAE) and xylanase activities were detected in culture supernatants from Humicola grisea var. thermoidea and Talaromyces stipitatus grown on brewers’ spent grain (BSG) and wheat bran (WB), two agro-industrial by-products. Maximum activities were detected from cultures of H. grisea grown at 150rpm, with 16.9U/ml and 9.1U/ml of xylanase activity on BSG and WB, respectively. Maximum FAE activity was 0.47U/ml and 0.33U/ml on BSG and WB, respectively. Analysis of residual cell wall material after microbial growth shows the preferential solubilisation of arabinoxylan and cellulose, two main polysaccharides present in BSG and WB. The production of low-cost cell-wall-deconstructing enzymes on agro-industrial by-products could lead to the production of low-cost enzymes for use in the valorisation of food processing wastes.