Effect of dietary fatty acids on Ca2+-ATPase activity of the sarcoplasmic reticulum of rainbow trout skeletal muscle
Effect of dietary fatty acids on Ca2+-ATPase activity of the sarcoplasmic reticulum of rainbow trout skeletal muscle
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DOI：
10.1016/s0305-0491(97)00229-0
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发表时间：
1997-11-01
影响因子：
2.2
通讯作者：
Watanabe, T
中科院分区：
文献类型：
--
作者：
Ushio, H;Ohshima, T;Watanabe, T
研究了玉米油、鱼肝油和牛脂对虹鳟肌浆网（SR）脂质组成和Ca2+- atp酶活性的影响。饲粮富含n-6脂肪酸或缺乏多不饱和脂肪酸（PUFA） 2个月后，肌肉和SR中磷脂酰乙醇胺（PE）水平低于饲粮富含n-3脂肪酸组。肌肉的脂肪酸组成很好地反映了饮食。另一方面，SRs比肌肉含有更高水平的多不饱和脂肪酸，如22:6n-3。特别是，各SR的PE组分中多不饱和脂肪酸含量非常高，饲喂pufa缺乏日粮3个月不能显著降低PE中22:6n-3的水平。磷脂酰胆碱组分的脂肪酸组成比磷脂酰胆碱组分更容易受到饲料脂肪酸的影响。富n-3脂肪酸日粮喂养导致SR Ca2+- atp酶活性降低。在实验阶段，Ca2+- atp酶蛋白的肽图谱几乎没有变化，这表明Ca2+- atp酶片段本身在初级结构中几乎没有变化。由此可见，饲料中脂肪酸组成会改变虹鳟鱼SRs的脂类和脂肪酸组成，从而导致脂质构象的改变。由此可见，脂质的这种生理变化可能会影响SR犊牛atp酶的活性。(C) 1997爱思唯尔科学有限公司
Effects of different dietary lipids, corn oil, cod liver oil, and beef tallow, on lipid compositions and Ca2+-ATPase activity of the rainbow trout Salmo gairdneri sarcoplasmic reticulum (SR) were examined. The levels of phosphatidylethanolamine (PE) of the muscle and the SR were lower in fish fed for 2 months on diets rich in n-6 fatty acids or deficient in polyunsaturated fatty acids (PUFA) than in those fed n-3 fatty acid rich diet. Fatty acid compositions of muscle well reflected those of the diets. On the other hand, the SRs contained higher levels of polyunsaturated fatty acids, such as 22:6n-3, than muscle. In particular, PE fractions of each SR exhibited very high contents of polyunsaturated fatty acids and feeding PUFA-deficient diet for 3 months failed to reduce markedly the level of 22:6n-3 in PEs. Fatty acid compositions of phosphatidylcholine (PC) fractions of the SRs were altered by dietary fatty acids more easily than those of PEs. n-3 Fatty acid rich diet feeding caused reduction of SR Ca2+-ATPase activities. There was hardly any difference in peptide mapping patterns of Ca2+-ATPase proteins through the experimental stages, suggesting that there was practically no change of the Ca2+-ATPase moiety itself in the primary structure. These results suggested that dietary fatty acid compositions would alter lipid class and fatty acid compositions of the rainbow trout SRs and consequently lipid conformation changes. It was concluded that such physiological changes in lipids might affect the SR Calf ATPase activities. (C) 1997 Elsevier Science Inc.