Comparison between conventional and molecular methods for diagnosis of bovine babesiosis (Babesia bovis infection) in tick infested cattle in upper Egypt.
Comparison between conventional and molecular methods for diagnosis of bovine babesiosis (Babesia bovis infection) in tick infested cattle in upper Egypt.
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DOI：
10.1007/s12639-016-0785-2
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发表时间：
2017-03-01
影响因子：
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通讯作者：
AL-Hosary, Amira A.T.
中科院分区：
文献类型：
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作者：
AL-Hosary, Amira A.T.
蜱和蜱传疾病是影响埃及畜牧业生产的主要问题。牛巴贝斯虫病对动物健康和生产造成严重影响。比较了Giemsa染色血涂片、聚合酶链反应（PCR）和巢式PCR（nPCR）检测埃及Baladi牛（Bos taurus）牛巴虫感染的方法与反向线性杂交方法。PCR和巢式PCR（nPCR）的敏感性分别为65%和100%. Giemsa染色血涂片显示最低的敏感性（30%）。根据这些结果，使用PCR和nPCR靶向B。BBOV-IV 005650（BV 5650）基因适合于B的诊断。牛传染病经18 Ss rRNA部分序列测定，所有阳性样品均为牛巴氏杆菌，并已在GenBank数据库中登录（登录号：KM 455548、KM 455549和KM 455550）。
Ticks and tick-borne diseases are the main problems affecting the livestock production in Egypt. Bovine babesiosis has adverse effects on the animal health and production. A comparison of Giemsa stained blood smears, polymerase chain reaction (PCR) and nested PCR (nPCR) assays for detection of Babesia bovis infection in Egyptian Baladi cattle (Bos taurus) in reference to reverse line blot was carried out. The sensitivity of PCR and nested PCR (nPCR) assays were 65 and 100 % respectively. Giemsa stained blood smears showed the lowest sensitivity (30 %). According to these results using of PCR and nPCR target for B. bovis, (BBOV-IV005650 (BV5650)) gene are suitable for diagnosis of B. bovis infection. The 18Ss rRNA partial sequence confirmed that all the positive samples were Babesia bovis and all of them were deposited in the GenBank databases (Accession No: KM455548, KM455549 and KM455550).