The characterization and immunoreactivity of a 70 kD protein common to Mycobacterium leprae and Mycobacterium bovis (BCG).
The characterization and immunoreactivity of a 70 kD protein common to Mycobacterium leprae and Mycobacterium bovis (BCG).
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麻风分枝杆菌和牛分枝杆菌 (BCG) 常见的 70 kD 蛋白质的特征和免疫反应性。
作者：
W. Britton;R. Garsia;L. Hellqvist;J. Watson;A. Basten
在麻风分枝杆菌和结核分枝杆菌的超声样品中鉴定出一种70 kD的蛋白抗原。牛（卡介苗）（BCG）与鼠单克隆抗体（Mab）。该抗原易于放射性标记，并由单克隆抗体，L7，或麻风血清免疫沉淀。在二维光谱上，它的pI值为5。1 .从BCG纯化后的蛋白质刺激来自Mantoux阳性受试者的淋巴细胞的增殖和淋巴因子分泌，并引起体内皮肤试验反应。测定了BCG蛋白的N-末端残基的序列。已从M.麻风病人
A 70 kD protein antigen has been identified in sonicates of Mycobacterium leprae and M. bovis (Baci l lus Cal mette-Guerin) (BCG) with murine monoclonal antibodies (Mabs) . The antigen was readily radiolabelled and was immunoprecipitated by the Mab , L 7, or leprosy sera . On two-dimensional electrophoresi s , it had a pI of 5 . 1 . The protein , after purification from BCG , stimulated prol iferation and lymphokine secretion in lymphocytes from Mantoux positive subjects and elicited skin test reactivity in vivo. The sequence of N-terminal res idues of the BCG protein was determined . Two DNA clones encoding the Mab-defined epitopes have been isolated from the genome of M. leprae.