Genome sequence of the recombinant protein production host Pichia pastoris
Genome sequence of the recombinant protein production host Pichia pastoris
复制标题
DOI：
10.1038/nbt.1544
复制
发表时间：
2009-06-01
影响因子：
46.9
通讯作者：
Callewaert, Nico
中科院分区：
文献类型：
--
作者：
De Schutter, Kristof;Lin, Yao-Cheng;Callewaert, Nico
甲醇营养型酵母巴斯德毕赤酵母被广泛用于蛋白质生产，并作为研究过氧化物酶体生物合成和甲醇同化的模式生物。能够进行人型N-糖基化的巴斯德毕赤酵母菌株现在是可获得的，这增加了该生物体用于生物制药生产的效用。尽管其生物技术的重要性，相对较少的遗传工具或工程菌株已产生的巴斯德毕赤酵母。为了促进这些领域的进展，我们提出了9.43 Mbp的基因组序列的GS 115菌株的巴斯德毕赤酵母。我们还为它的5，313个蛋白质编码基因提供了手动管理的注释。
The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes.