Gene expression of connective tissue growth factor (CTGF/CCN2) in calcifying tissues of normal and cbfa1-null mutant mice in late stage of embryonic development
Gene expression of connective tissue growth factor (CTGF/CCN2) in calcifying tissues of normal and cbfa1-null mutant mice in late stage of embryonic development
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DOI：
10.1007/s00774-004-0600-5
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发表时间：
2005-07-01
影响因子：
3.3
通讯作者：
Takigawa, M
中科院分区：
文献类型：
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作者：
Yamaai, T;Nakanishi, T;Takigawa, M
结缔组织生长因子 (CTGF/CCN2) 是最近描述的生长因子之一，由软骨细胞、血管内皮细胞和转化生长因子 (TGF)-β 刺激的成纤维细胞产生。 CTGF 是从软骨肉瘤来源的软骨细胞系 HCS-2/8 中分离出来的，发现它在软骨组织中正常表达，特别是在肥大软骨细胞中，并且在体外刺激软骨细胞的增殖和分化。因此，CTGF被认为是体内软骨内骨化最重要的调节因子之一。在此，我们描述了正常发育小鼠胚胎钙化组织中 ctgf 基因的表达模式，并与核心结合因子 a1 (Cbfa1) 靶向突变 (cbfa1-null) 小鼠胚胎中的表达模式进行比较，其中在骨骼系统中观察到典型的发育和生长受损。发育15天后（E15），在肥大和临时钙化区域检测到ctgf的表达，在小鼠胚胎骨骼发育过程中，该区域骨化向骨骺进行。此外，ctgf 在围产期左右发育中的磨牙和切牙胚中表达。然而，在 cbfa1 缺失的小鼠胚胎中没有发现该基因的表达。这些结果表明CTGF可能在小鼠胚胎钙化组织的发育中具有某些重要作用。
Connective tissue growth factor (CTGF/CCN2), one of the most recently described growth factors, is produced by chondrocytes, vascular endothelial cells, and transforming growth factor (TGF)-beta-stimulated fibroblasts. CTGF was isolated from a chondrosarcoma-derived chondrocytic cell line, HCS-2/8, and found to be normally expressed in cartilage tissues, especially in hypertrophic chondrocytes, and also to stimulate both the proliferation and the differentiation of chondrocytes in vitro. Therefore, CTGF is thought to be one of the most important regulators of endochondral ossification in vivo. Herein we describe the expression pattern of the ctgf gene in the calcifying tissues of normal developing mouse embryos in comparison with that in core binding factor a1 (Cbfa1)-targeted mutant (cbfa1-null) mouse embryos, in which impaired development and growth were characteristically observed in the skeletal system. After 15 days of development (E15), the expression of ctgf was detected in the zone of hypertrophy and provisional calcification, in which ossification proceeds toward the epiphysis during the skeletal development of the mouse embryo. Furthermore, ctgf was expressed in developing molar and incisal tooth germs around the perinatal stage. However, no expression of the gene was found in the cbfa1-null mouse embryos. These results indicate that CTGF may have certain important roles in the development of the calcifying tissues in the mouse embryo.