GOLGI STUDY OF GOLDFISH OPTIC TECTUM
GOLGI STUDY OF GOLDFISH OPTIC TECTUM
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DOI：
10.1002/cne.901820107
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发表时间：
1978-01-01
影响因子：
2.5
通讯作者：
SCHELLART, NAM
中科院分区：
文献类型：
--
作者：
MEEK, J;SCHELLART, NAM
本文用快速高尔基法、高尔基-Kopsch法和改良的高尔基-Cox法对金鱼视顶盖进行了研究。根据树突树和轴突特性的位置，区分了15种细胞类型。发现两种细胞类型的树突在边缘层：I型与轴突终止于中央灰质层和II型没有浸渍轴突。三种细胞类型（III，IV和V）在一个单一的，特定的顶盖层和轴突终止于顶盖内的树突。五种细胞类型（VI-X）在2个水平面中具有树突。两个有髓鞘的轴突离开顶盖，而其余3个项目的轴突到不同的顶盖层。虽然这前10种细胞类型几乎只在顶盖的表面一半有树突，但其余5种类型在更深层也有树突。这尤其适用于最明显的顶盖细胞（XII型和XIII型），它们具有在3个或更多水平上分支的树突树和离开顶盖的有髓轴突，有时具有非常特殊的路线（XIII 1）。在顶盖最深层（XIV型）的大量细胞和胞体中，除了最浅和最深层外，在顶盖的任何地方都有树突和轴突终末。大多数树突发生在视神经层，而轴突，总是起源于树突轴在表层顶盖的一半，一般终止于中间顶盖层。XV型细胞的索马也位于顶盖的最深层，但树突不能到达视神经层。每个顶盖叶估计有以下数量：I型，5000- 20，000个神经元; III型，250- 10，000个; IV-XIII型，各500-2000个; XIV型，1，000，000 - 2，000，000个。有髓顶盖传出神经的总数估计为2000-8000。通过与其他硬骨鱼类高尔基体研究的比较，可以得出结论：这些鱼类的覆盖层基本相似。
A study of goldfish [Carassius auratus] optic tectum was performed with rapid-Golgi, Golgi-Kopsch and a modified Golgi-Cox impregnation which proved quite suitable to impregnate cells in the middle tectal layers and to study more closely axonal properties. Fifteen cell types are distinguished, based upon the position of dendritic trees and axonal properties. Two cell types are found with dendrites in the marginal layer: type I with an axon terminating in the central gray layer and type II without an impregnated axon. Three cell types (III, IV and V) have dendrites in a single, specific tectal layer and an axon terminating within the tectum. Five cell types (VI-X) have dendrites in 2 horizontal planes. Two have myelinated axons leaving the tectum, whereas the axons of the remaining 3 project to different tectal layers. While these first 10 cell types have dendrites almost exclusively in the superficial half of the tectum, the remaining 5 types also have dendrites in deeper layers. This especially holds for the most conspicuous tectal cells (types XII and XIII), which have dendritic trees branching at 3 or more horizontal levels and a myelinated axon leaving the tectum, with sometimes a very peculiar course (XIII1). Also, type XI has 3 or more dendritic trees, but its axon was not found. The numerous cells and cell bodies in the deepest tectal layer (type XIV) have dendrites and axonal terminations anywhere in the tectum, except in the most superficial and the deepest layer. Most dendrites occur in the optic layers, whereas the axons, always originating from the dendritic shaft in the superficial tectal half, generally terminate in the middle tectal layers. Type XV cells have their soma in the deepest tectal layer as well, but their dendrites do not reach the optic layers. Per tectal lobe the following numbers are estimated: type I, 5000-20,000 neurons; Type III, 250-10,000; types IV-XIII, each 500-2000 and type XIV, 1,000,000-2,000,000. The total number of myelinated tectal efferents is estimated at 2000-8000. Comparison with other Golgi studies in teleosts leads to the conclusion that the tecta of these species of fish are basically similar.