Use of ultrasound-targeted microbubble destruction to transfect IGF-1 cDNA to enhance the regeneration of rat wounded Achilles tendon in vivo
Use of ultrasound-targeted microbubble destruction to transfect IGF-1 cDNA to enhance the regeneration of rat wounded Achilles tendon in vivo
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DOI：
10.1038/gt.2015.32
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发表时间：
2015-04
期刊：
影响因子：
5.1
通讯作者：
Yuanjiao Tang;Qianying Leng;Xi Xiang;Ling-yan Zhang;Yu-jia Yang;Li Qiu
中科院分区：
文献类型：
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作者：
Yuanjiao Tang;Qianying Leng;Xi Xiang;Ling-yan Zhang;Yu-jia Yang;Li Qiu
本研究的目的是确定是否使用超声靶向微泡破坏（UTMD），以胰岛素样生长因子-1（IGF-1）cDNA转染大鼠跟腱损伤，将促进组织再生。将40只大鼠跟腱损伤后随机分为对照组、单纯质粒组、质粒+超声组和质粒+微泡+超声组。采用组织学粘连观察、实时荧光定量PCR检测和生物力学测试等方法检测跟腱组织中IGF-1cDNA的表达。第4组术后2周和8周的粘连评分最低（P< 0.05）。跟腱IGF-1的表达在第2周和第8周以第4组最高（P< 0.05）。与其他3组相比，第2周时，第4组的肉芽组织和炎性细胞浸润较轻，第8周时，第4组肌腱上的瘢痕较不明显。第4组IGF-1 mRNA在第2周和第8周表达明显上调（P< 0.01）。第4组和第3组的最大载荷、刚度和极限应力均高于对照组（P< 0.05）。第4组跟腱愈合后的最大载荷、刚度和极限应力在第2周和第8周最高（P< 0.05）。
The purpose of this study was to determine whether using ultrasound-targeted microbubble destruction (UTMD) to transfect rat wounded Achilles tendon with insulin-like growth factor-1 (IGF-1) cDNA would enhance tissue regeneration. Forty rats with injured Achilles tendons were transfected with IGF-1 cDNA and divided into:(1) control group,(2) plasmid-only group,(3) plasmid+ ultrasound group and (4) plasmid+ microbubbles+ ultrasound group. The IGF-1 cDNA expression of the Achilles tendons was evaluated by histological adhesion finding, quantitative real-time reverse transcription PCR examination and biomechanical test. The adhesion scores in group 4 were lowest at weeks 2 and 8 (P< 0.05). The IGF-1 expression in the Achilles tendons was highest in group 4 at weeks 2 and 8 (P< 0.05). Compared with those of other three groups, the granulation tissues and inflammatory-cell infiltration were lighter in group 4 at week 2, and the scars on the tendons in group 4 were less evident at week 8. The messenger RNA (mRNA) of IGF-1 of group 4 was upregulated at weeks 2 and 8 (P< 0.01). Groups 4 and 3 showed a greater maximum load, stiffness and ultimate stress (P< 0.05). Maximum load, stiffness and ultimate stress of healing Achilles tendons in group 4 were highest at weeks 2 and 8 (P< 0.05).