The EC-HDA9 complex rhythmically regulates histone acetylation at the TOC1 promoter in Arabidopsis
The EC-HDA9 complex rhythmically regulates histone acetylation at the TOC1 promoter in Arabidopsis
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DOI：
10.1038/s42003-019-0377-7
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发表时间：
2019-04-23
影响因子：
5.9
通讯作者：
Seo, Pil Joon
中科院分区：
文献类型：
--
作者：
Lee, Kyounghee;Mas, Paloma;Seo, Pil Joon
生物钟是一些原核生物和高等真核生物中保守的计时机制。染色质修饰正在成为改善核心时钟基因表达的关键调控机制。组蛋白标记的节律性变化与拟南芥时钟基因中CAB表达的时间安排1（TOC 1）密切相关。然而，负责这些标记的染色质相关修饰剂仍然在很大程度上未知。在这里，我们发现，染色质修饰剂组蛋白去乙酰化酶9（HDA 9）和晚上复合物（EC）组件EARLY FLOWER 3（ELF 3）直接相互作用，以调节TOC 1在其峰值表达后的下降阶段。我们发现HDA 9通过与ELF 3的相互作用特异性结合TOC 1启动子。EC-HDA 9复合物促进TOC 1位点的H3脱乙酰化，有助于在EC功能的夜间抑制TOC 1表达。因此，我们已经确定的机制，昼夜节律钟交织与染色质相关的组件，以塑造基因表达的昼夜节律波形在拟南芥。
Circadian clocks are conserved time-keeper mechanisms in some prokaryotes and higher eukaryotes. Chromatin modification is emerging as key regulatory mechanism for refining core clock gene expression. Rhythmic changes in histone marks are closely associated to the TIMING OF CAB EXPRESSION 1 (TOC1) Arabidopsis clock gene. However, the chromatin-related modifiers responsible for these marks remain largely unknown. Here, we uncover that the chromatin modifier HISTONE DEACETYLASE 9 (HDA9) and the Evening complex (EC) component EARLY FLOWERING 3 (ELF3) directly interact to regulate the declining phase of TOC1 after its peak expression. We found that HDA9 specifically binds to the TOC1 promoter through the interaction with ELF3. The EC-HDA9 complex promotes H3 deacetylation at the TOC1 locus, contributing to suppressing TOC1 expression during the night, the time of EC function. Therefore, we have identified the mechanism by which the circadian clock intertwines with chromatin-related components to shape the circadian waveforms of gene expression in Arabidopsis.