NERVE GROWTH-FACTOR STIMULATION OF THE RAS-GUANINE NUCLEOTIDE EXCHANGE FACTOR AND GAP ACTIVITIES
NERVE GROWTH-FACTOR STIMULATION OF THE RAS-GUANINE NUCLEOTIDE EXCHANGE FACTOR AND GAP ACTIVITIES
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DOI：
10.1126/science.1604323
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发表时间：
1992-06-05
期刊：
影响因子：
56.9
通讯作者：
KAMATA, T
中科院分区：
文献类型：
--
作者：
LI, BQ;KAPLAN, D;KAMATA, T
Ras蛋白的生物活性被认为是由鸟嘌呤核苷酸交换因子和鸟苷三磷酸酶激活蛋白（GAP）控制的。用神经生长因子（NGF）处理大鼠嗜铬细胞瘤PC-12细胞，增加了活性Ras三磷酸鸟苷复合物的量，并刺激了鸟嘌呤核苷酸交换因子和GAP的活性。在PC-12细胞中，过表达的酪氨酸激酶编码的trk原癌基因（高亲和力的神经生长因子受体的一个组成部分），神经生长因子诱导的激活的调节蛋白增强。这些结果表明，神经生长因子受体系统增强的鸟嘌呤核苷酸交换因子和GAP的活动和Ras的激活可能是由这两个调节蛋白之间的活性平衡控制。
The biological activity of Ras proteins is thought to be controlled by the guanine nucleotide exchange factor and the guanosine triphosphatase activating protein (GAP). Treatment of rat pheochromocytoma PC-12 cells with nerve growth factor (NGF) increased the amount of active Ras guanosine triphosphate complex and stimulated the activities of both the guanine nucleotide exchange factor and GAP. In PC-12 cells that overexpressed the tyrosine kinase encoded by the trk proto-oncogene (a component of the high-affinity NGF receptor), the NGF-induced activation of the regulatory proteins was potentiated. These results suggest that the NGF receptor system enhances the activities of both the guanine nucleotide exchange factor and GAP and that the activation of Ras might be controlled by the balance in activity between these two regulatory proteins.