The Residual Innate Lymphoid Cells in NFIL3-Deficient Mice Support Suboptimal Maternal Adaptations to Pregnancy.
The Residual Innate Lymphoid Cells in NFIL3-Deficient Mice Support Suboptimal Maternal Adaptations to Pregnancy.
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DOI：
10.3389/fimmu.2016.00043
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发表时间：
2016
影响因子：
7.3
通讯作者：
Colucci F
中科院分区：
文献类型：
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作者：
Boulenouar S;Doisne JM;Sferruzzi-Perri A;Gaynor LM;Kieckbusch J;Balmas E;Yung HW;Javadzadeh S;Volmer L;Hawkes DA;Phillips K;Brady HJ;Fowden AL;Burton GJ;Moffett A;Colucci F
子宫NK细胞是先天淋巴样细胞（ILC），在怀孕期间填充子宫并扩张，调节人类和小鼠胎盘发育和胎儿生长。我们最近表征了子宫ILCs （uILCs）的组成，其中一些需要转录因子NFIL3，但NFIL3依赖性细胞在小鼠中支持成功繁殖的程度尚不清楚。通过将Nfil3−/−雌性与野生型雄性交配，我们发现母体细胞中Nfil3缺乏对怀孕期间uILCs的变化以及对生殖的下游影响。尽管在怀孕的Nfil3 - / -小鼠中存在CD49a+Eomes - uILC1s，并且CD49a+Eomes+组织内NK细胞和uILC3s的残余大量增加，我们发现在正常大小的窝中，子宫动脉和蜕膜的不完全重塑、胎盘缺陷和胎儿生长受限。这些结果表明，母源NFIL3介导小鼠生殖中的非冗余功能。
Uterine NK cells are innate lymphoid cells (ILC) that populate the uterus and expand during pregnancy, regulating placental development and fetal growth in humans and mice. We have recently characterized the composition of uterine ILCs (uILCs), some of which require the transcription factor NFIL3, but the extent to which NFIL3-dependent cells support successful reproduction in mice is unknown. By mating Nfil3−/− females with wild-type males, here we show the effects of NFIL3 deficiency in maternal cells on both the changes in uILCs during pregnancy and the downstream consequences on reproduction. Despite the presence of CD49a+Eomes− uILC1s and the considerable expansion of residual CD49a+Eomes+ tissue-resident NK cells and uILC3s in pregnant Nfil3−/− mice, we found incomplete remodeling of uterine arteries and decidua, placental defects, and fetal growth restriction in litters of normal size. These results show that maternal NFIL3 mediates non-redundant functions in mouse reproduction.