Inhibition of myoblast migration by prostacyclin is associated with enhanced cell fusion
Inhibition of myoblast migration by prostacyclin is associated with enhanced cell fusion
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DOI：
10.1096/fj.06-7070com
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发表时间：
2007-10-01
期刊：
影响因子：
4.8
通讯作者：
Pavlath, Grace K.
中科院分区：
文献类型：
--
作者：
Bondesen, Brenda A.;Jones, Kristen A.;Pavlath, Grace K.
卫星细胞是在肌发生过程中对骨骼肌的形成和生长至关重要的干细胞。为了分化和融合，增殖的卫星细胞或成肌细胞必须迁移并建立稳定的细胞-细胞接触。然而，调控成肌细胞迁移和融合的因素还不完全清楚。我们已经确定PGI（2）是一种新的体外肌生成调节因子。PGI（2）是前列腺素（PG）家族的一员，是通过环氧合酶-1和-2途径合成的自分泌/旁分泌信号分子。原代小鼠肌细胞在肌发生的不同阶段既分泌PGI（2）又表达PGI（2）受体IP。利用遗传学和药理学方法，我们发现PGI（2）是成肌细胞迁移的负调节因子，也能促进细胞融合。因此，PGI（2）可以作为迁移细胞的“制动器”，以促进细胞-细胞接触和融合。总之，我们的研究结果强调了细胞迁移和肌生成中正调节因子和负调节因子之间平衡的重要性。这项工作可能对其他群体的成体干细胞和/或经历融合的细胞的迁移有影响。邦德森，B。一、琼斯，K.一、格拉斯哥，W. C.的方法，Pavlath，G. K.前列环素对成肌细胞迁移的抑制与增强的细胞融合有关。
Satellite cells are stem cells that are critical for the formation and growth of skeletal muscle during myogenesis. To differentiate and fuse, proliferating satellite cells or myoblasts must migrate and establish stable cell-cell contacts. However, the factors that regulate myoblast migration and fusion are not understood completely. We have identified PGI(2) as a novel regulator of myogenesis in vitro. PGI(2) is a member of the family of prostaglandins ( PG), autocrine/paracrine signaling molecules synthesized via the cyclo-oxygenase-1 and -2 pathways. Primary mouse muscle cells both secrete PGI(2) and express the PGI(2) receptor, IP, at various stages of myogenesis. Using genetic and pharmacological approaches, we show that PGI(2) is a negative regulator of myoblast migration that also enhances cell fusion. Thus, PGI(2) may act as a "brake" on migrating cells to facilitate cell-cell contact and fusion. Together, our results highlight the importance of the balance between positive and negative regulators in cell migration and myogenesis. This work may have implications for migration of other populations of adult stem cells and/or cells that undergo fusion. Bondesen, B. A., Jones, K. A., Glasgow, W. C., Pavlath, G. K. Inhibition of myoblast migration by prostacyclin is associated with enhanced cell fusion.